Anti-PGP9.5 antibody [BH7] (ab72911)
![Flow Cytometry - Anti-PGP9.5 antibody [BH7] (ab72911)](https://www.abcam.com/ps/products/72/ab72911/Images/ab72911-200113-anti-pgp95-antibody-flow-cytometry.jpg "Flow Cytometry - Anti-PGP9.5 antibody [BH7] (ab72911)")
Key features and details
- Mouse monoclonal [BH7] to PGP9.5
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-PGP9.5 antibody [BH7]
See all PGP9.5 primary antibodies -
Description
Mouse monoclonal [BH7] to PGP9.5 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length human PGP9.5 purified from E. coli.
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Positive control
- Rat hippocampal cell homogenate and rat spinal cord tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.065% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
BH7 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 25 kDa).
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| ICC/IF |
1/500.
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| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
|
WB
1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 25 kDa). |
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ICC/IF
1/500. |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. -
Tissue specificity
Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. -
Involvement in disease
Parkinson disease 5
Neurodegeneration with optic atrophy, childhood-onset -
Sequence similarities
Belongs to the peptidase C12 family. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain. - Information by UniProt
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Database links
- Entrez Gene: 7345 Human
- Entrez Gene: 29545 Rat
- Omim: 191342 Human
- SwissProt: P09936 Human
- SwissProt: Q00981 Rat
- Unigene: 518731 Human
- Unigene: 107213 Rat
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Alternative names
- Epididymis luminal protein 117 antibody
- Epididymis secretory protein Li 53 antibody
- HEL 117 antibody
see all
Images
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Flow Cytometry - Anti-PGP9.5 antibody [BH7] (ab72911)Overlay histogram showing SH-SY5Y cells stained with ab72911 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab72911, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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![Western blot - Anti-PGP9.5 antibody [BH7] (ab72911)](https://www.abcam.com/ps/products/72/ab72911/Images/ab72911-88147-anti-pgp95-antibody-western-blot.gif)
Western blot - Anti-PGP9.5 antibody [BH7] (ab72911)Anti-PGP9.5 antibody [BH7] (ab72911) at 1/5000 dilution + rat hippocampal homogenate
Predicted band size: 25 kDa
Observed band size: <43 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody [BH7] (ab72911)](https://www.abcam.com/ps/products/72/ab72911/Images/ab72911-88146-anti-pgp95-antibody-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody [BH7] (ab72911)ab72911 at 1/500 dilution staining PGP9.5 in rat spinal cord by Immunofluorescence (shown in red). Anti-neurofilament NF-H antibody staining is shown in green. The large cells are a-motorneurons and PGP9.5 fills the cytoplasm of their perikarya and dendrites.
Protocols
Datasheets and documents
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Datasheet download
References (9)
ab72911 has been referenced in 9 publications.
- Bloom ML et al. Renewal and Differentiation of GCD Necklace Olfactory Sensory Neurons. Chem Senses 45:333-346 (2020). PubMed: 32333759
- Gray M et al. Development and characterisation of acquired radioresistant breast cancer cell lines. Radiat Oncol 14:64 (2019). PubMed: 30987655
- Donadio V et al. The autonomic innervation of hairy skin in humans: an in vivo confocal study. Sci Rep 9:16982 (2019). PubMed: 31740757
- Sowa Y et al. Direct Conversion of Human Fibroblasts into Schwann Cells that Facilitate Regeneration of Injured Peripheral Nerve In Vivo. Stem Cells Transl Med 6:1207-1216 (2017). PubMed: 28186702
- Axer H et al. The impairment of small nerve fibers in severe sepsis and septic shock. Crit Care 20:64 (2016). PubMed: 26984636
- Dong H et al. Inhibitory motor neurons of the esophageal myenteric plexus are mechanosensitive. Am J Physiol Cell Physiol 308:C405-13 (2015). PubMed: 25540174
- Dong H et al. Morphological, immunocytochemical, and functional characterization of esophageal enteric neurons in primary culture. Am J Physiol Gastrointest Liver Physiol 305:G129-38 (2013). PubMed: 23660501
- Verkerk AO et al. Functional Nav1.8 channels in intracardiac neurons: the link between SCN10A and cardiac electrophysiology. Circ Res 111:333-43 (2012). PubMed: 22723301
- Siman R et al. Evidence that a Panel of Neurodegeneration Biomarkers Predicts Vasospasm, Infarction, and Outcome in Aneurysmal Subarachnoid Hemorrhage. PLoS One 6:e28938 (2011). WB ; Human . PubMed: 22174930
