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  1. Link

    pgp95-antibody-bh7-ab72911.pdf

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Neuroscience Neurology process Neurodegenerative disease Parkinson's disease Parkin / PARK
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Anti-PGP9.5 antibody [BH7] (ab72911)

  • Datasheet
Submit a review Submit a question References (13)

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Flow Cytometry - Anti-PGP9.5 antibody [BH7] (ab72911)
  • Western blot - Anti-PGP9.5 antibody [BH7] (ab72911)
  • Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody [BH7] (ab72911)

Key features and details

  • Mouse monoclonal [BH7] to PGP9.5
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-PGP9.5 antibody [BH7]
    See all PGP9.5 primary antibodies
  • Description

    Mouse monoclonal [BH7] to PGP9.5
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length human PGP9.5 purified from E. coli.

  • Positive control

    • Rat hippocampal cell homogenate and rat spinal cord tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.065% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    BH7
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Parkin / PARK
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Deubiquitination
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Neuronal
    • Neuroscience
    • Diseases

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant human PGP9.5 protein (ab53962)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab72911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 25 kDa).
ICC/IF
1/500.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Notes
WB
1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 25 kDa).
ICC/IF
1/500.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • Tissue specificity

    Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
  • Involvement in disease

    Parkinson disease 5
    Neurodegeneration with optic atrophy, childhood-onset
  • Sequence similarities

    Belongs to the peptidase C12 family.
  • Post-translational
    modifications

    O-glycosylated.
  • Cellular localization

    Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
  • Target information above from: UniProt accession P09936 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7345 Human
    • Entrez Gene: 29545 Rat
    • Omim: 191342 Human
    • SwissProt: P09936 Human
    • SwissProt: Q00981 Rat
    • Unigene: 518731 Human
    • Unigene: 107213 Rat
    • Alternative names

      • Epididymis luminal protein 117 antibody
      • Epididymis secretory protein Li 53 antibody
      • HEL 117 antibody
      • HEL S 53 antibody
      • NDGOA antibody
      • Neuron cytoplasmic protein 9.5 antibody
      • OTTHUMP00000218137 antibody
      • OTTHUMP00000218139 antibody
      • OTTHUMP00000218140 antibody
      • OTTHUMP00000218141 antibody
      • Park 5 antibody
      • PARK5 antibody
      • PGP 9.5 antibody
      • PGP9.5 antibody
      • PGP95 antibody
      • Protein gene product 9.5 antibody
      • Ubiquitin C terminal esterase L1 antibody
      • Ubiquitin C terminal hydrolase antibody
      • Ubiquitin C terminal hydrolase L1 antibody
      • Ubiquitin carboxyl terminal esterase L1 antibody
      • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
      • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
      • Ubiquitin thioesterase L1 antibody
      • Ubiquitin thiolesterase antibody
      • Ubiquitin thiolesterase L1 antibody
      • UCH-L1 antibody
      • UCHL1 antibody
      • UCHL1_HUMAN antibody
      see all

    Images

    • Flow Cytometry - Anti-PGP9.5 antibody [BH7] (ab72911)
      Flow Cytometry - Anti-PGP9.5 antibody [BH7] (ab72911)
      Overlay histogram showing SH-SY5Y cells stained with ab72911 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab72911, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Western blot - Anti-PGP9.5 antibody [BH7] (ab72911)
      Western blot - Anti-PGP9.5 antibody [BH7] (ab72911)
      Anti-PGP9.5 antibody [BH7] (ab72911) at 1/5000 dilution + rat hippocampal homogenate

      Predicted band size: 25 kDa
      Observed band size: <43 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody [BH7] (ab72911)
      Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody [BH7] (ab72911)
      ab72911 at 1/500 dilution staining PGP9.5 in rat spinal cord by Immunofluorescence (shown in red). Anti-neurofilament NF-H antibody staining is shown in green. The large cells are a-motorneurons and PGP9.5 fills the cytoplasm of their perikarya and dendrites.

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (13)

    Publishing research using ab72911? Please let us know so that we can cite the reference in this datasheet.

    ab72911 has been referenced in 13 publications.

    • Alhilou AM  et al. Density of nerve fibres and expression of substance P, NR2B-receptors and nerve growth factor in healthy human masseter muscle: An immunohistochemical study. J Oral Rehabil 48:35-44 (2021). PubMed: 33031568
    • Donadio V  et al. In Vivo Diagnosis of Synucleinopathies: A Comparative Study of Skin Biopsy and RT-QuIC. Neurology 96:e2513-e2524 (2021). PubMed: 33837116
    • Donadio V  et al. Skin Biopsy May Help to Distinguish Multiple System Atrophy-Parkinsonism from Parkinson's Disease With Orthostatic Hypotension. Mov Disord 35:1649-1657 (2020). PubMed: 32557839
    • Bloom ML  et al. Renewal and Differentiation of GCD Necklace Olfactory Sensory Neurons. Chem Senses 45:333-346 (2020). PubMed: 32333759
    • Gray M  et al. Development and characterisation of acquired radioresistant breast cancer cell lines. Radiat Oncol 14:64 (2019). PubMed: 30987655
    View all Publications for this product

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