Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal to PGP9.5 - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Mouse, Human
Product nameAnti-PGP9.5 antibody - BSA and Azide free (Capture)
See all PGP9.5 primary antibodies
DescriptionRabbit monoclonal to PGP9.5 - BSA and Azide free (Capture)
Tested applicationsSuitable for: Sandwich ELISAmore details
Species reactivityReacts with: Mouse, Human
ab244697 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Mouse UCHL1. The recommended pair for sandwich ELISA is:
Capture: ab244697, Human/Mouse UCHL1 Capture Antibody (unconjugated)
Detector: ab244963, Human/Mouse UCHL1 Detector Antibody (unconjugated)
The reference range value is 313 - 20000 pg/ml for human and 156.3 - 10000 pg/ml for mouse.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
-High batch-to-batch consistency and reproducibility
-Improved sensitivity and specificity
-Long-term security of supply
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituent: 100% PBS
Concentration information loading...
sELISA pair antibody
Our Abpromise guarantee covers the use of ab244697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal to PGP9.5 - BSA and Azide free (Detector) (ab244963).|
FunctionUbiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Tissue specificityFound in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
Involvement in diseaseParkinson disease 5
Neurodegeneration with optic atrophy, childhood-onset
Sequence similaritiesBelongs to the peptidase C12 family.
Cellular localizationCytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
- Information by UniProt
- Epididymis luminal protein 117 antibody
- Epididymis secretory protein Li 53 antibody
- HEL 117 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244697 has not yet been referenced specifically in any publications.