Validated using a knockout cell line

Anti-PGP9.5 antibody [EPR4117] (ab109261)


  • Product name
    Anti-PGP9.5 antibody [EPR4117]
    See all PGP9.5 primary antibodies
  • Description
    Rabbit monoclonal [EPR4117] to PGP9.5
  • Host species
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PGP9.5 aa 50-150. The exact sequence is proprietary.

  • Positive control
    • Fetal brain, Y79, U-87 MG, HAP1 and SH SY5Y lysates; Human brain tissue.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab109261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform antigen retrieval before commencing with IHC staining protocol
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
    • Tissue specificity
      Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
    • Involvement in disease
      Parkinson disease 5
      Neurodegeneration with optic atrophy, childhood-onset
    • Sequence similarities
      Belongs to the peptidase C12 family.
    • Post-translational
    • Cellular localization
      Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Epididymis luminal protein 117 antibody
      • Epididymis secretory protein Li 53 antibody
      • HEL 117 antibody
      • HEL S 53 antibody
      • NDGOA antibody
      • Neuron cytoplasmic protein 9.5 antibody
      • OTTHUMP00000218137 antibody
      • OTTHUMP00000218139 antibody
      • OTTHUMP00000218140 antibody
      • OTTHUMP00000218141 antibody
      • Park 5 antibody
      • PARK5 antibody
      • PGP 9.5 antibody
      • PGP9.5 antibody
      • PGP95 antibody
      • Protein gene product 9.5 antibody
      • Ubiquitin C terminal esterase L1 antibody
      • Ubiquitin C terminal hydrolase antibody
      • Ubiquitin C terminal hydrolase L1 antibody
      • Ubiquitin carboxyl terminal esterase L1 antibody
      • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
      • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
      • Ubiquitin thioesterase L1 antibody
      • Ubiquitin thiolesterase antibody
      • Ubiquitin thiolesterase L1 antibody
      • UCH-L1 antibody
      • UCHL1 antibody
      • UCHL1_HUMAN antibody
      see all


    • Lane 1: Wild type HAP1 whole cell lysate (20 µg)              
      Lane 2: UCHL1 (KO) knockout  HAP1 whole cell lysate (20 µg)
      Lane 3: SH-SY5Y whole cell lysate (20 µg)
      Lane 4: HEK293 whole cell lysate (20 µg)              

      Lanes 1 - 4: Merged signal (red and green). Green - ab109261 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

      Ab109261 was shown to specifically react with UCHL1 (KO) in wild-type cells as signal was lost in UCHL1 (KO) knockout HAP1 cells. Wild-type and UCHL1 (KO) knockout samples were subjected to SDS-PAGE.  Ab109261 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • ab109261 at 1/50 dilution staining PGP9.5 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.
    • All lanes : Anti-PGP9.5 antibody [EPR4117] (ab109261) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Y79 lysate
      Lane 3 : U-87 MG lysate
      Lane 4 : SH SY5Y lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 24 kDa

    • All lanes : Anti-PGP9.5 antibody [EPR4117] (ab109261) at 1/1000 dilution

      Lane 1 : Whole tissue lysate prepared from adult mouse brain homogenate (CD-1 strain)
      Lane 2 : Whole tissue lysate prepared from non transfected control cells (low endogenous expression of UCH-L1)
      Lane 3 : Whole tissue lysate prepared from cells transfected with UCH-L1-Flag cDNA (positive control)

      Lysates/proteins at 100 µg per lane.

      All lanes : HRP conjugated goat anti-rabiit polyclonal at 1/10000 dilution

      Developed using the ECL technique.

      Predicted band size: 24 kDa

      Exposure time: 2 minutes

      See Abreview

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    This product has been referenced in:
    • Purmessur D  et al. Notochordal cell-derived therapeutic strategies for discogenic back pain. Global Spine J 3:201-18 (2013). IHC . Read more (PubMed: 24436871) »

    See 1 Publication for this product

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