Overview

  • Product name

    Anti-PGP9.5 antibody [EPR4117] - BSA and Azide free
    See all PGP9.5 primary antibodies
  • Description

    Rabbit monoclonal [EPR4117] to PGP9.5 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PGP9.5 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Fetal brain, Y79, U-87 MG, HAP1 and SH SY5Y lysates; Human brain tissue.
  • General notes

    Ab176838 is the carrier-free version of ab109261. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab176838 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab176838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
    • Tissue specificity

      Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
    • Involvement in disease

      Parkinson disease 5
      Neurodegeneration with optic atrophy, childhood-onset
    • Sequence similarities

      Belongs to the peptidase C12 family.
    • Post-translational
      modifications

      O-glycosylated.
    • Cellular localization

      Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
    • Information by UniProt
    • Database links

    • Alternative names

      • Epididymis luminal protein 117 antibody
      • Epididymis secretory protein Li 53 antibody
      • HEL 117 antibody
      • HEL S 53 antibody
      • NDGOA antibody
      • Neuron cytoplasmic protein 9.5 antibody
      • OTTHUMP00000218137 antibody
      • OTTHUMP00000218139 antibody
      • OTTHUMP00000218140 antibody
      • OTTHUMP00000218141 antibody
      • Park 5 antibody
      • PARK5 antibody
      • PGP 9.5 antibody
      • PGP9.5 antibody
      • PGP95 antibody
      • Protein gene product 9.5 antibody
      • Ubiquitin C terminal esterase L1 antibody
      • Ubiquitin C terminal hydrolase antibody
      • Ubiquitin C terminal hydrolase L1 antibody
      • Ubiquitin carboxyl terminal esterase L1 antibody
      • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
      • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
      • Ubiquitin thioesterase L1 antibody
      • Ubiquitin thiolesterase antibody
      • Ubiquitin thiolesterase L1 antibody
      • UCH-L1 antibody
      • UCHL1 antibody
      • UCHL1_HUMAN antibody
      see all

    Images

    • This IHC data was generated using the same anti-PGP9.5 antibody clone, EPR4117, in a different buffer formulation (cat# ab109261).

      ab109261 at 1/50 dilution staining PGP9.5 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • This WB data was generated using the same anti-PGP9.5 antibody clone, EPR4117, in a different buffer formulation (cat# ab109261).

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)              
      Lane 2: UCHL1 (KO) knockout  HAP1 whole cell lysate (20 µg)
      Lane 3: SH-SY5Y whole cell lysate (20 µg)
      Lane 4: HEK293 whole cell lysate (20 µg)              

      Lanes 1 - 4: Merged signal (red and green). Green - ab109261 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109261 was shown to specifically react with UCHL1 (KO) in wild-type cells as signal was lost in UCHL1 (KO) knockout HAP1 cells. Wild-type and UCHL1 (KO) knockout samples were subjected to SDS-PAGE. ab109261 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    References

    ab176838 has not yet been referenced specifically in any publications.

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