Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PGP9.5 antibody - Neuronal Marker

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0
Sample
Rat Tissue sections (Dorsal root ganglion)
Specification
Dorsal root ganglion
Permeabilization
Yes - 0,1% Tween -20
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: +4°C · Diluent: TBS, 0,1% Tween 20, 2% Normal goat serum
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Anti-guinea pig IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 568
Dilution
1/500

Additional data

Additional Notes
We also tried another antigen retrieval protocol and it the antibody aslo worked fine.

Yulia Sidorova

Verified customer

Submitted Feb 03 2014

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