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    pgp95-antibody-ruchl1775-ab238074.pdf

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Neuroscience Neurology process Neurodegenerative disease Parkinson's disease Parkin / PARK
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Recombinant

Recombinant Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)
  • Western blot - Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)
  • Protein Array - Anti-PGP9.5 antibody (ab238074)
  • Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [rUCHL1/775] to PGP9.5
  • Suitable for: Protein Array, WB, IHC-P
  • Reacts with: Human

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Recombinant human PGP9.5 protein (ab53962)

View more associated products

Overview

  • Product name

    Anti-PGP9.5 antibody [rUCHL1/775]
    See all PGP9.5 primary antibodies
  • Description

    Mouse monoclonal [rUCHL1/775] to PGP9.5
  • Host species

    Mouse
  • Tested applications

    Suitable for: Protein Array, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human PGP9.5.
    Database link: P09936

  • Positive control

    • IHC-P: Human brain tissue. WB: Human brain tissue lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from bioreactor concentrate.
  • Clonality

    Monoclonal
  • Clone number

    rUCHL1/775
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Parkin / PARK
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Deubiquitination
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Neuronal
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP kits
    • Neuroscience
    • Diseases

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Recombinant Protein

    • Recombinant human PGP9.5 protein (ab53962)
  • Related Products

    • Recombinant human PGP9.5 protein (ab198431)
    • Recombinant Mouse PGP9.5 protein (ab202233)
    • Recombinant human PGP9.5 protein (ab53962)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab238074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 24 kDa.
IHC-P
1/1 - 1/2.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 mins followed by cooling at RT for 20 mins. Incubate with for 30 minutes at RT.

Notes
Protein Array
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 24 kDa.
IHC-P
1/1 - 1/2.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 mins followed by cooling at RT for 20 mins. Incubate with for 30 minutes at RT.

Target

  • Function

    Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • Tissue specificity

    Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
  • Involvement in disease

    Parkinson disease 5
    Neurodegeneration with optic atrophy, childhood-onset
  • Sequence similarities

    Belongs to the peptidase C12 family.
  • Post-translational
    modifications

    O-glycosylated.
  • Cellular localization

    Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
  • Target information above from: UniProt accession P09936 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7345 Human
    • Omim: 191342 Human
    • SwissProt: P09936 Human
    • Unigene: 518731 Human
    • Alternative names

      • Epididymis luminal protein 117 antibody
      • Epididymis secretory protein Li 53 antibody
      • HEL 117 antibody
      • HEL S 53 antibody
      • NDGOA antibody
      • Neuron cytoplasmic protein 9.5 antibody
      • OTTHUMP00000218137 antibody
      • OTTHUMP00000218139 antibody
      • OTTHUMP00000218140 antibody
      • OTTHUMP00000218141 antibody
      • Park 5 antibody
      • PARK5 antibody
      • PGP 9.5 antibody
      • PGP9.5 antibody
      • PGP95 antibody
      • Protein gene product 9.5 antibody
      • Ubiquitin C terminal esterase L1 antibody
      • Ubiquitin C terminal hydrolase antibody
      • Ubiquitin C terminal hydrolase L1 antibody
      • Ubiquitin carboxyl terminal esterase L1 antibody
      • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
      • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
      • Ubiquitin thioesterase L1 antibody
      • Ubiquitin thiolesterase antibody
      • Ubiquitin thiolesterase L1 antibody
      • UCH-L1 antibody
      • UCHL1 antibody
      • UCHL1_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)

      Formalin-fixed, paraffin-embedded human pancreas tissue stained for PGP9.5 with ab238074 at 2 µg/ml in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)

      Formalin-fixed, paraffin-embedded human brain tissue stained for PGP9.5 with ab238074 at 2 µg/ml in immunohistochemical analysis.

    • Western blot - Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)
      Western blot - Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)
      Anti-PGP9.5 antibody [rUCHL1/775] (ab238074) at 2 µg/ml + Human brain tissue lysate

      Predicted band size: 24 kDa

    • Protein Array - Anti-PGP9.5 antibody (ab238074)
      Protein Array - Anti-PGP9.5 antibody (ab238074)
      ab238074 was tested in protein array against over 19000 different full-length human proteins.
      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
      A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
    • Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)
      Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab238074? Please let us know so that we can cite the reference in this datasheet.

    ab238074 has not yet been referenced specifically in any publications.

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