Recombinant Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)](https://www.abcam.com/ps/products/238/ab238074/Images/ab238074-342988-Mouse-monoclonal-rUCHL1-775-to-PGP95-immunohistochemistry-pancreas-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [rUCHL1/775] to PGP9.5
- Suitable for: Protein Array, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-PGP9.5 antibody [rUCHL1/775]
See all PGP9.5 primary antibodies -
Description
Mouse monoclonal [rUCHL1/775] to PGP9.5 -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human PGP9.5.
Database link: P09936 -
Positive control
- IHC-P: Human brain tissue. WB: Human brain tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
rUCHL1/775 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Protein Array |
Use at an assay dependent concentration.
|
|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 24 kDa.
|
|
| IHC-P |
1/1 - 1/2.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 mins followed by cooling at RT for 20 mins. Incubate with for 30 minutes at RT. |
| Notes |
|---|
|
Protein Array
Use at an assay dependent concentration. |
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 24 kDa. |
|
IHC-P
1/1 - 1/2. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 mins followed by cooling at RT for 20 mins. Incubate with for 30 minutes at RT. |
Target
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Function
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. -
Tissue specificity
Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. -
Involvement in disease
Parkinson disease 5
Neurodegeneration with optic atrophy, childhood-onset -
Sequence similarities
Belongs to the peptidase C12 family. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain. - Information by UniProt
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Database links
- Entrez Gene: 7345 Human
- Omim: 191342 Human
- SwissProt: P09936 Human
- Unigene: 518731 Human
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Alternative names
- Epididymis luminal protein 117 antibody
- Epididymis secretory protein Li 53 antibody
- HEL 117 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)
Formalin-fixed, paraffin-embedded human pancreas tissue stained for PGP9.5 with ab238074 at 2 µg/ml in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)](https://www.abcam.com/ps/products/238/ab238074/Images/ab238074-342987-Mouse-monoclonal-rUCHL1-775-to-PGP95-immunohistochemistry-brain-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)Formalin-fixed, paraffin-embedded human brain tissue stained for PGP9.5 with ab238074 at 2 µg/ml in immunohistochemical analysis.
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![Western blot - Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)](https://www.abcam.com/ps/products/238/ab238074/Images/ab238074-342986-Mouse-monoclonal-rUCHL1-775-to-PGP95-western-blot-brain-human1.jpg)
Western blot - Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)Anti-PGP9.5 antibody [rUCHL1/775] (ab238074) at 2 µg/ml + Human brain tissue lysate
Predicted band size: 24 kDa -
Protein Array - Anti-PGP9.5 antibody (ab238074)ab238074 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. -
![Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)](https://www.abcam.com/ps/products/238/ab238074/Images/ab238074-5-benefits-of-recombinant-antibodies.png)
Anti-PGP9.5 antibody [rUCHL1/775] (ab238074)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab238074 has not yet been referenced specifically in any publications.
