Anti-PGRMC1 antibody (ab224054)
Key features and details
- Rabbit polyclonal to PGRMC1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PGRMC1 antibody
See all PGRMC1 primary antibodies -
Description
Rabbit polyclonal to PGRMC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig, Orangutan -
Immunogen
Recombinant fragment corresponding to Human PGRMC1 aa 1-150.
Database link: O00264 -
Positive control
- IHC-P: Human liver, kidney, endometrium, hippocampus tissue and breast cancer tissue. WB: NIH/3T3, NBT-II and HEK-293 cell lysate. Human liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 22 kDa.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 22 kDa. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for progesterone. -
Tissue specificity
Widely expressed, with highest expression in liver and kidney. -
Sequence similarities
Belongs to the cytochrome b5 family. MAPR subfamily.
Contains 1 cytochrome b5 heme-binding domain. -
Domain
The cytochrome b5 heme-binding domain lacks the conserved iron-binding His residues at positions 107 and 131. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 317706 Cow
- Entrez Gene: 10857 Human
- Entrez Gene: 53328 Mouse
- Entrez Gene: 100174262 Orangutan
- Entrez Gene: 396946 Pig
- Entrez Gene: 291948 Rat
- Omim: 300435 Human
- SwissProt: Q17QC0 Cow
see all -
Alternative names
- HPR6.6 antibody
- Membrane associated progesterone receptor component 1 antibody
- Membrane-associated progesterone receptor component 1 antibody
see all
Images
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Anti-PGRMC1 antibody (ab224054) + Human Liver tissue
Predicted band size: 22 kDa -
Paraffin embedded human endometrium tissue stained for PGRMC1 with ab224054 (1/500 dilution) in immunohistochemical analysis.
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Anti-PGRMC1 antibody (ab224054) at 1/100 dilution + HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Predicted band size: 22 kDa -
All lanes : Anti-PGRMC1 antibody (ab224054) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : NBT-II (rat cell line) cell lysate
Predicted band size: 22 kDa -
Negative control: Paraffin embedded human skeletal tissue stained for PGRMC1 with ab224054 (1/500 dilution) in immunohistochemical analysis.
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Paraffin embedded human liver tissue stained for PGRMC1 with ab224054 (1/500 dilution) in immunohistochemical analysis.
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Paraffin embedded human kidney tissue stained for PGRMC1 with ab224054 (1/500 dilution) in immunohistochemical analysis.
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Paraffin embedded human breast cancer tissue stained for PGRMC1 with ab224054 (1/500 dilution) in immunohistochemical analysis.
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Paraffin embedded human hippocampus tissue stained for PGRMC1 with ab224054 (1/500 dilution) in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab224054 has not yet been referenced specifically in any publications.