Key features and details
- Rabbit polyclonal to PGRMC1 - C-terminal
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PGRMC1 antibody - C-terminal
See all PGRMC1 primary antibodies
DescriptionRabbit polyclonal to PGRMC1 - C-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Sheep, Rabbit, Horse, Cow, Cat, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan
- HeLa, Jurkat, 293T and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab194879 was affinity purified using an epitope specific to PGRMC1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab194879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 22 kDa.|
FunctionReceptor for progesterone.
Tissue specificityWidely expressed, with highest expression in liver and kidney.
Sequence similaritiesBelongs to the cytochrome b5 family. MAPR subfamily.
Contains 1 cytochrome b5 heme-binding domain.
DomainThe cytochrome b5 heme-binding domain lacks the conserved iron-binding His residues at positions 107 and 131.
Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
- Information by UniProt
- HPR6.6 antibody
- Membrane associated progesterone receptor component 1 antibody
- Membrane-associated progesterone receptor component 1 antibody
All lanes : Anti-PGRMC1 antibody - C-terminal (ab194879) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 22 kDa
Exposure time: 30 seconds
Lysates in lanes 1-3 were prepared using RIPA lysis buffer. Lysates in lanes 4-5 were prepared using NETN lysis buffer.
Detection of PGRMC1 in immunoprecipitates of 293T whole cell lysate prepared using RIPA lysis buffer (1 mg for IP, 20% of IP loaded) using ab194879 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab194879 has not yet been referenced specifically in any publications.