Overview

  • Product name
    Anti-PGRPS antibody [188C424]
    See all PGRPS primary antibodies
  • Description
    Mouse monoclonal [188C424] to PGRPS
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide: YVLKGHRDVQRTLSPG , corresponding to amino acids 165-180 of Human PGRPS.

  • Positive control
    • Tested with mouse spleen tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab13903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notes
    WB: Use at a concentration of 2 µg/ml. Predicted molecular weight: 22 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria. Has bactericidal activity towards Gram-positive bacteria. May kill Gram-positive bacteria by interfering with peptidoglycan biosynthesis. Binds also to Gram-negative bacteria, and has bacteriostatic activity towards Gram-negative bacteria. Plays a role in innate immunity.
    • Tissue specificity
      Highly expressed in bone marrow. Weak expression found in kidney, liver, small intestine, spleen, thymus, peripheral leukocyte, lung, fetal spleen and neutrophils.
    • Sequence similarities
      Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.
    • Post-translational
      modifications
      N-glycosylated. N-glycosylation is required for bactericidal activity.
    • Cellular localization
      Secreted. Cytoplasmic granule.
    • Information by UniProt
    • Database links
    • Alternative names
      • MGC126894 antibody
      • MGC126896 antibody
      • Peptidoglycan recognition protein 1 antibody
      • Peptidoglycan recognition protein antibody
      • Peptidoglycan recognition protein short antibody
      • PGLYRP antibody
      • PGLYRP1 antibody
      • PGRP antibody
      • PGRP S antibody
      • PGRP-S antibody
      • PGRP1_HUMAN antibody
      • PHRP, short antibody
      • SBBI68 antibody
      • TAG7 antibody
      • TNF superfamily, member 3 (LTB)-like (peptidoglycan recognition protein) antibody
      • TNFSF 3L antibody
      • TNFSF3L antibody
      • UNQ639/PRO1269 antibody
      see all

    Images

    • ab13903 at 1/100 staining human bone Marrow derived CD34+ cells by ICC/IF. The cells were paraformaldehyde fixed, permeabilized and blocked with BSA before incubation with the antibody for 24 hours at 4°C. A FITC conjugated goat anti-mouse IgG was used as the secondary. The cells were counterstained with DAPI.

      The image shows staining mainly in the cytoplasmic region.

      See Abreview

    • Anti-PGRPS antibody [188C424] (ab13903) at 2 µg/ml + Jurkat cell lysate

      Predicted band size: 22 kDa
      Observed band size: 28 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:
    • Dey S & Bishayi B Riboflavin along with antibiotics balances reactive oxygen species and inflammatory cytokines and controls Staphylococcus aureus infection by boosting murine macrophage function and regulates inflammation. J Inflamm (Lond) 13:36 (2016). IF . Read more (PubMed: 27932936) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Bone Marrow derived CD34+ cells)
    Specification
    Bone Marrow derived CD34+ cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.25% Triton X-100 for 10min
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Jun 29 2007

    Question

    Following further investigation into the antibody ab13903 (anti-PGRPS), I have further questions. Although the product sheet listed the positive control as mouse spleen lysate and although you apparently confirmed this in your first reply, the paper by Liu et al (JBC 275(32):24490, 2000) shows no expression of PGRPS by western blotting of a mouse spleen lysate!!(Figure 8). Since ab13903 is a mouse monoclonal, a secondary to mouse IgG will also detect the immunoglobulin light chain at 25 kDa from B cells in the spleen. Since this is close to the 20 kDa of the PGRPS does the strong signal from the mouse Ig light chain interfere with the 20 kDa PGRPS? Does one have to run a non-reducing western? The figure on the Imgenex site is more in line with 25 kDa than 20 kDa and there is no appropriate negative control. In a related question, I cannot find a publication that reports using this monoclonal to detect PGRPS. Both papers from Liu on the data sheet use polyclonal rabbit antisera. I did not see a specific PGRPS signal in a human PMN lysate nor a mouse spleen lysate (see comment above) although my positive control (p38) worked very well. Any information would be appreciated. If you cannot provide me with any more compelling information about the positive controls and proven function of ab13903, I would be interested in exchanging this antibody for any other polyclonal anti-PGRPS antibodies from Abcam (though I cannot locate one in your search function) or another antibody that you do offer. Please comment on this issue.

    Read More
    Answer

    Thank you for your enquiry. Unfortunately I do not have access to the Lui paper. According to the Swiss Prot reference for mouse the target is “strongly expressed in spleen”, this was the positive control that was used. The predicted molecular weight, according to the Swiss Prot entry would be 23.5 KDa. You are correct that the KDa of the light chain of IgG is approximately 20 KDa, however it is unlikely that interference would be seen from the IgG produced by B cells, it is more likely that the entire KDa of the IgG1 would be seen at approximately 146 KDa. A number of secondary Abs are available: If the secondary Ab is Fab specific it will cross react with both the heavy and the light chain of the Ab structure. If working with tissues or cells that have Fc receptors (spleen) a certain amount of cross reactivity will be seen unless a F(ab )2 is used to eliminate non-specific binding through Fc receptors present on cells. Alternatively, Fc receptors can be blocked. Perform an absorption step, using purified IgG from the host species of the your secondary antibody. In that case an F(ab )2 secondary antibody may not be necessary. Alternatively kits are available that are capable of directly biotinylating the primary monoclonal Ab that is being used (e.g. Dako) and thus eliminating the need to use a anti mouse secondary. The references that are cited on the product data sheet are not specific references, but general references that have been added in order to help provide further back ground information, as such they do not specifically quote the product. We hope this information will be useful for you.

    Read More

    Answer

    Thank you for your enquiry. This product will cross react with mouse, the data sheet has now been updated t reflect this. Western blot analysis was carried out on PGRP-S in tissue lysates from mouse spleen using Ab13903 at a dilution of 2 µg/ml. Thank you for brining this matter to my attention, if you require any further information then please do not hesitate to get back in touch.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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