Anti-PGRPS antibody (ab232728)
Key features and details
- Rabbit polyclonal to PGRPS
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-PGRPS antibody
See all PGRPS primary antibodies -
Description
Rabbit polyclonal to PGRPS -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Rat PGRPS aa 1 to the C-terminus. Two N-terminal tags, (Expressed in E.coli).
Database link: Q9JLN4 -
Positive control
- WB: Human urine; Recombinant rat PGRPS protein. IHC-P: Rat spleen tissue. ICC/IF: HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.5 - 5 µg/ml.
|
|
| ICC/IF |
Use a concentration of 5 - 20 µg/ml.
|
|
| IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 0.5 - 5 µg/ml. |
|
ICC/IF
Use a concentration of 5 - 20 µg/ml. |
|
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
-
Function
Pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria. Has bactericidal activity towards Gram-positive bacteria. May kill Gram-positive bacteria by interfering with peptidoglycan biosynthesis. Binds also to Gram-negative bacteria, and has bacteriostatic activity towards Gram-negative bacteria. Plays a role in innate immunity. -
Tissue specificity
Highly expressed in bone marrow. Weak expression found in kidney, liver, small intestine, spleen, thymus, peripheral leukocyte, lung, fetal spleen and neutrophils. -
Sequence similarities
Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family. -
Post-translational
modificationsN-glycosylated. N-glycosylation is required for bactericidal activity. -
Cellular localization
Secreted. Cytoplasmic granule. - Information by UniProt
-
Database links
- Entrez Gene: 8993 Human
- Entrez Gene: 84387 Rat
- Omim: 604963 Human
- SwissProt: O75594 Human
- SwissProt: Q9JLN4 Rat
- Unigene: 137583 Human
- Unigene: 2834 Rat
-
Alternative names
- MGC126894 antibody
- MGC126896 antibody
- Peptidoglycan recognition protein 1 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-PGRPS antibody (ab232728)
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PGRPS using ab232728 at 20 µg/mL in ICC/IF. FITC staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGRPS antibody (ab232728)Formalin-fixed, paraffin-embedded rat spleen tissue stained for PGRPS using ab232728 at 20 µg/mL in immunohistochemical analysis. DAB staining.
-
Western blot - Anti-PGRPS antibody (ab232728)Anti-PGRPS antibody (ab232728) at 3 µg/ml + Human urine
Secondary
HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution -
Western blot - Anti-PGRPS antibody (ab232728)Anti-PGRPS antibody (ab232728) at 3 µg/ml + Recombinant rat PGRPS protein
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab232728 has not yet been referenced specifically in any publications.