Overview

  • Product name

    Anti-PH2 antibody
  • Description

    Rabbit polyclonal to PH2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human PH2 aa 553-835.
    Database link: Q8IXK0

  • Positive control

    • HEK293 and MCF7 lysates
  • General notes

    Previously labelled as HDC2 / PHC2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab124405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 36, 30 kDa (predicted molecular weight: 91,88,36,30 kDa).

Target

  • Function

    Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
  • Sequence similarities

    Contains 1 FCS-type zinc finger.
    Contains 1 SAM (sterile alpha motif) domain.
  • Domain

    HD1 motif interacts with SAM domain of PHC1.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Form

    There are 5 isoforms produced by alternative splicing.
  • Alternative names

    • EDR2 antibody
    • early development regulator 2 (homolog of polyhomeotic 2) antibody
    • early development regulator 2 like antibody
    • Early development regulatory protein 2 antibody
    • HDC2 / PHC2 antibody
    • hPH2 antibody
    • MGC163502 antibody
    • mph2 antibody
    • PH2 antibody
    • PHC2 antibody
    • PHC2_HUMAN antibody
    • polyhomeotic 2 antibody
    • polyhomeotic like 2 antibody
    • Polyhomeotic-like protein 2 antibody
    see all

Images

References

ab124405 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
50 µg
Treatment
2i and LIF
Specification
Mouse embryonic stem cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Sep 15 2015

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