Key features and details
- Rabbit polyclonal to PH3
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PH3 antibody
See all PH3 primary antibodies
DescriptionRabbit polyclonal to PH3
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human PH3 aa 933-983. The exact sequence is proprietary.
Database link: Q8NDX5
- IP: HeLa whole cell lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Use at 2-5 µg/mg of lysate.
FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Sequence similaritiesContains 1 FCS-type zinc finger.
Contains 1 SAM (sterile alpha motif) domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Early development regulator 3 antibody
- Early development regulatory protein 3 antibody
- EDR3 antibody
PH3 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264212 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different antibody.
Lane 1: ab264212 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 3 mins.
Cells prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264212 has not yet been referenced specifically in any publications.