Product nameAnti-PH3 antibody
See all PH3 primary antibodies
DescriptionRabbit polyclonal to PH3
Tested applicationsSuitable for: ICC/IF, WB, IPmore details
Species reactivityReacts with: Human, Drosophila melanogaster
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide within Human PH3 aa 700-750. The exact sequence is proprietary.
Database link: AAI31773.1
- HeLa whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified using an epitope specific to PH3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab80612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 22019301|
|WB||1/2000 - 1/10000. Predicted molecular weight: 106 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Sequence similaritiesContains 1 FCS-type zinc finger.
Contains 1 SAM (sterile alpha motif) domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
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All lanes : Anti-PH3 antibody (ab80612) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 106 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Immunoprecipitation of HeLa whole cell lysate using ab80612 at 3µg/mg lysate (lane 1) or a control IgG (lane 2). Bands were detected by Western blot using ab80612 at 1µg/ml. Chemiluminescence with exposure times of 3 minutes.
This product has been referenced in:
- Datta RR et al. A dissection of the teashirt and tiptop genes reveals a novel mechanism for regulating transcription factor activity. Dev Biol 360:391-402 (2011). ICC/IF ; Drosophila melanogaster . Read more (PubMed: 22019301) »