Overview

  • Product name

  • Description

    Rabbit polyclonal to PHACTR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Zebrafish
  • Immunogen

    Synthetic peptide designed within residues: QRPTAEELEQ RNILKPRNEQ EEQEEKREIK RRLTRKLSQR PTVEELRERK, corresponding to internal sequence amino acids 468-517 of Human PHACTR1 (NP_112210)

  • Positive control

    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-PHACTR1 antibody (ab84646) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size: 66 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PHACTR1 with ab84646 at 5.0µg/ml.

References

This product has been referenced in:

  • Zhang Z  et al. PHACTR1 regulates oxidative stress and inflammation to coronary artery endothelial cells via interaction with NF-?B/p65. Atherosclerosis 278:180-189 (2018). Read more (PubMed: 30293016) »
  • Reschen ME  et al. Genetic and environmental risk factors for atherosclerosis regulate transcription of phosphatase and actin regulating gene PHACTR1. Atherosclerosis 250:95-105 (2016). IHC-P ; Human . Read more (PubMed: 27187934) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Ventana Protease 1
Permeabilization
No
Specification
Brain
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 25 2018

Answer

Thank you for your enquiry and your interest in our products.
I can confirm that lysates from non-transfected Jurkat cells were used to test this antibody (ab84646) for Western blot application.
PHACTR1 is expressed in bone marrow and lymph node, you may wish to visit UniProt website for further information:
http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.436996
If you need any further assistance, please do not hesitate to contact me.

Read More

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