Anti-PHACTR1 antibody (ab84646)

Reviews (1)Q&A (1)References (2)

Overview

  • Product name

    Anti-PHACTR1 antibody
    See all PHACTR1 primary antibodies

  • Description

    Rabbit polyclonal to PHACTR1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Zebrafish

  • Immunogen

    Synthetic peptide designed within residues: QRPTAEELEQ RNILKPRNEQ EEQEEKREIK RRLTRKLSQR PTVEELRERK, corresponding to internal sequence amino acids 468-517 of Human PHACTR1 (NP_112210)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Western blot - Anti-PHACTR1 antibody (ab84646)
    Western blot - Anti-PHACTR1 antibody (ab84646)
    Anti-PHACTR1 antibody (ab84646) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size: 66 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHACTR1 antibody (ab84646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHACTR1 antibody (ab84646)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PHACTR1 with ab84646 at 5.0µg/ml.

References

This product has been referenced in:

  • Zhang Z  et al. PHACTR1 regulates oxidative stress and inflammation to coronary artery endothelial cells via interaction with NF-?B/p65. Atherosclerosis 278:180-189 (2018). Read more (PubMed: 30293016) »
  • Reschen ME  et al. Genetic and environmental risk factors for atherosclerosis regulate transcription of phosphatase and actin regulating gene PHACTR1. Atherosclerosis 250:95-105 (2016). IHC-P ; Human . Read more (PubMed: 27187934) »
See all 2 Publications for this product

Publishing research using ab84646? Please let us know so that we can cite the reference in this datasheet.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PHACTR1 antibody

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Ventana Protease 1
Permeabilization
No
Specification
Brain
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde
Read More

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Verified customer

Submitted Sep 25 2018

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