Overview

  • Product name

    Phagocytosis Assay Kit (Green Zymosan)
    See all Phagocytosis kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Phagocytosis Assay Kit (Green Zymosan) (ab234053) utilizes pre-labeled Zymosan particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. The kit provides a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like receptors ligands (TLR).

  • Notes

    Phagocytosis in mammals serves as an important first line defense mechanism against invading pathogens. It is also essential for continuous clearance of dying cells, tissue remodeling, and acquisition of nutrients for some cells. Phagocytosis is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. Zymosan prepared from yeast cell wall (Saccharomyces cerevisiae), and consisting of protein-carbohydrate complexes is frequently used as a pathogen in phagocytosis assays.

  • Platform

    Microplate reader, Fluor. microscope, Flow cyt.

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 100 tests
    Phagocytosis Assay Buffer 2 x 100ml
    Buffer Additive 2 x 1ml
    Green Zymosan (FITC) 1 x 600µl
    10X Quenching Solution 1 x 500µl

Images

  • J774 macrophages were seeded overnight at 5 x 105 of viable cells/well. The next day the cells were pretreated with 20 µM Cytochalasin D for 1 h at 37ºC prior to addition of 5 µL of Zymosan particles. Phagocytosis was conducted for 2 hours and the amount of engulfed Zymosan was determined as described in the Assay Protocol. Inhibition of phagocytosis. Panel A and B: images of non-treated cells. Panel C and D: treatment with Cytochalasin D.

  • J774 macrophages were seeded overnight at 5 x 105 of viable cells/well. The next day the cells were pretreated with 20 µM Cytochalasin D for 1 h at 37ºC prior to addition of 5 µL of Zymosan particles. Phagocytosis was conducted for 2 hours and the amount of engulfed Zymosan was determined as described in the Assay Protocol. Flow cytometry plot. Red line: untreated control cells; green line: macrophages with engulfed Zymosan particles; blue line: inhibition of phagocytosis by Cytochalasin D.

Protocols

References

ab234053 has not yet been referenced specifically in any publications.

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