Key features and details
- Assay type: Cell-based (qualitative)
- Detection method: Fluorescent
- Platform: Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Product namePhalloidin FITC Reagent
See all F-actin kits
Sample typeAdherent cells, Suspension cells
Assay typeCell-based (qualitative)
Phalloidin FITC Reagent (ab235137) is one of a series of phalloidin conjugates that selectively binds to actin filaments, also known as F-actin.
Phalloidin conjugates are convenient probes for labeling, identifying and quantitating animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.
Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 488, Phalloidin-iFluor 647, Phalloidin-iFluor 594, Phalloidin-iFluor 555, and Rhodamine Phalloidin, search the website to see all phallodin conjugates, or read the phalloidin staining protocol.
Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.
When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 300 tests Phalloidin FITC Conjugate 1 x 300 tests
- actin filament
- f actin
- Filamentous actin
ab235137 has been referenced in 3 publications.
- Najmi Z et al. Evaluation of Nisin and LL-37 Antimicrobial Peptides as Tool to Preserve Articular Cartilage Healing in a Septic Environment. Front Bioeng Biotechnol 8:561 (2020). PubMed: 32596225
- Li S et al. hBMSC-Derived Extracellular Vesicles Attenuate IL-1ß-Induced Catabolic Effects on OA-Chondrocytes by Regulating Pro-inflammatory Signaling Pathways. Front Bioeng Biotechnol 8:603598 (2020). PubMed: 33425869
- Li N et al. Vascular extracellular matrix and fibroblasts-coculture directed differentiation of human mesenchymal stem cells toward smooth muscle-like cells for vascular tissue engineering. Mater Sci Eng C Mater Biol Appl 93:61-69 (2018). PubMed: 30274093