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    phalloidin-ifluor-350-reagent-ab176751.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin
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Phalloidin-iFluor 350 Reagent (ab176751)

  • Datasheet
  • SDS
  • Protocol Booklet
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CytoPainter Phalloidin-iFluor 350 Reagent (ab176751)
  • CytoPainter Phalloidin-iFluor 350 Reagent (ab176751)

Key features and details

  • Assay type: Cell-based (qualitative)
  • Platform: Fluorescence microscope
  • Sample type: Adherent cells, Suspension cells

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Overview

  • Product name

    Phalloidin-iFluor 350 Reagent
    See all F-actin kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based (qualitative)
  • Product overview

    Phalloidin-iFluor 350 Reagent (ab176751) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-iFluor 350 can be easily detected with a fluorescent microscope at Ex/Em = 353/442 nm.


    Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.


    Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 488, Phalloidin-iFluor 647, Phalloidin-iFluor 594, Phalloidin-iFluor 555, and Rhodamine Phalloidin, search the website to see all phallodin conjugates, or read the phalloidin staining protocol.

  • Notes

    Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.

    When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.

  • Platform

    Fluorescence microscope

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 300 tests
    Phalloidin-iFluor 350 Conjugate 1 x 300 tests
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Kits/ Lysates/ Other
    • Kits
    • Cell Staining Kits
    • F-actin Staining Kits
  • Alternative names

    • actin filament
    • f actin
    • Filamentous actin

Images

  • CytoPainter Phalloidin-iFluor 350 Reagent (ab176751)
    CytoPainter Phalloidin-iFluor 350 Reagent (ab176751)

    Actin filaments staining in HeLa cells. Actin filaments (blue) were stained with CytoPainter Phalloidin-iFluor 350 reagent (ab176751).

  • CytoPainter Phalloidin-iFluor 350 Reagent (ab176751)
    CytoPainter Phalloidin-iFluor 350 Reagent (ab176751)

    Excitation and emission spectra of phalloidin-iFluor 350 reagent.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab176751? Please let us know so that we can cite the reference in this datasheet.

ab176751 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Staining for actin in mouse embryonic fibroblasts

Good Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
The product worked well for mouse embryonic fibroblasts at a dilution of 1:100 and an incubation time of 30 mins. This review is submitted as required by the terms of discount code #96248
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Avathamsa Athirasala

Verified customer

Submitted Dec 08 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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