Phalloidin-iFluor 350 Reagent (ab176751)
- Datasheet
- References
- Protocols
Overview
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Product name
Phalloidin-iFluor 350 Reagent
See all F-actin kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Product overview
Phalloidin-iFluor 350 Reagent (ab176751) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-iFluor 350 can be easily detected with a fluorescent microscope at Ex/Em = 353/442 nm.
Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.
Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 488, Phalloidin-iFluor 647, Phalloidin-iFluor 594, Phalloidin-iFluor 555, and Rhodamine Phalloidin, search the website to see all phallodin conjugates, or read the phalloidin staining protocol.
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Notes
Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.
When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 300 tests Phalloidin-iFluor 350 Conjugate 1 x 300 tests -
Research areas
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Alternative names
- actin filament
- f actin
- Filamentous actin
Images
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CytoPainter Phalloidin-iFluor 350 Reagent (ab176751)Actin filaments staining in HeLa cells. Actin filaments (blue) were stained with CytoPainter Phalloidin-iFluor 350 reagent (ab176751).
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CytoPainter Phalloidin-iFluor 350 Reagent (ab176751)Excitation and emission spectra of phalloidin-iFluor 350 reagent.
Protocols
References
ab176751 has not yet been referenced specifically in any publications.
