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Phalloidin-iFluor 488 Reagent (ab176753)

Reviews (13) Submit a question References (124)
Whole Mount Immunofluorescence of Mouse Inner Ear
  • Phalloidin iFluor 488 staining of macrophages
  • CytoPainter Phalloidin-iFluor 488 Reagent (ab176753)
  • CytoPainter Phalloidin-iFluor 488 Reagent (ab176753)

Key features and details

  • Assay type: Cell-based (qualitative)
  • Platform: Fluorescence microscope
  • Sample type: Adherent cells, Suspension cells

Overview

  • Product name

    Phalloidin-iFluor 488 Reagent
    See all F-actin kits

  • Sample type

    Adherent cells, Suspension cells

  • Assay type

    Cell-based (qualitative)

  • Product overview

    Phalloidin-iFluor 488 Reagent ab176753 is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. The iFluor 488 dye can be easily detected with a fluorescent microscope at Ex/Em = 493/517 nm.


    Our phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used with paraffin-embedded samples that have been de-paraffinized.


    Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 647, Phalloidin-iFluor 594, Phalloidin-iFluor 555, and Rhodamine Phalloidin, search the website to see all phallodin conjugates, or read the phalloidin staining protocol.

  • Notes

    Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.

    When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.

  • Platform

    Fluorescence microscope

Properties

Images

  • Whole Mount Immunofluorescence of Mouse Inner Ear
    Whole Mount Immunofluorescence of Mouse Inner EarImage courtesy of Mr. Shahar Taiber

    Ab176753 at 1/1000 dilution in PBS whole mount Immunofluorescence of Mouse inner ear sensory epithelia. Tissue was incubated for 2 hours at room temperature.

  • Phalloidin iFluor 488 staining of macrophages
    Phalloidin iFluor 488 staining of macrophagesReview by Dr. Jose Ramos Vivas

    Macrophage J774A.1 cell line stained with Phalloidin 488 and DAPI nuclear counterstaining.

  • CytoPainter Phalloidin-iFluor 488 Reagent (ab176753)
    CytoPainter Phalloidin-iFluor 488 Reagent (ab176753)

    Actin filaments staining in HeLa cells. Actin filaments (green) were stained with CytoPainter Phalloidin-iFluor 488 reagent (ab176753); tubulin filaments were stained with a mouse anti-tubulin antibody/goat anti-mouse IgG (red). Nuclei were stained with Hoechst 33342.

  • CytoPainter Phalloidin-iFluor 488 Reagent (ab176753)
    CytoPainter Phalloidin-iFluor 488 Reagent (ab176753)

    Excitation and emission spectra of phalloidin-iFluor 488 reagent.

References (124)

Publishing research using ab176753? Please let us know so that we can cite the reference in this datasheet.

ab176753 has been referenced in 124 publications.

  • Dewan SMR  et al. Complement C5a-triggered differentiated HL-60 stimulates migration of THP-1 monocytic leukocytes via secretion of CCL2. FEBS Open Bio N/A:N/A (2021). PubMed: 33715310
  • Zhang J  et al. Primary human colonic mucosal barrier crosstalk with super oxygen-sensitive Faecalibacterium prausnitzii in continuous culture. Med (N Y) 2:74-98.e9 (2021). PubMed: 33511375
  • Rashed FB  et al. Identification of proteins and cellular pathways targeted by 2-nitroimidazole hypoxic cytotoxins. Redox Biol 41:101905 (2021). PubMed: 33640700
  • Nagamachi A  et al. Multiorgan failure with abnormal receptor metabolism in mice mimicking Samd9/9L syndromes. J Clin Invest 131:N/A (2021). PubMed: 33373325
  • Bagher M  et al. Crosstalk between Mast Cells and Lung Fibroblasts Is Modified by Alveolar Extracellular Matrix and Influences Epithelial Migration. Int J Mol Sci 22:N/A (2021). PubMed: 33419174
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 13 Abreviews or Q&A

Phalloidin-FITC

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
Tested on A549 cells, dilution 1:2000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 19 2021

Phalloidin for IF of various human cells in culture

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
Has worked for a variety of cells in culture (BeWo choriocarcinoma, HTR8-SVNeo cells) with ease. Permeabilization using 0.1% TritonX-100 in PBS for 5 min necessary prior to incubation with phalloidin (1:1000 in 0.1%BSA in PBS) for 20 min at room temperature.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 21 2021

Phalloidin iFluor 488 staining of equine tenocytes

 Good Excellent 5/5 (Ease of Use)
Abreviews
abreview image
Images of unstimulated equine adult tenocytes on gelatin-coated coverslips. Cells were fixed in 4% PFA, permeabilized with Triton X (0.1%) for 60 min, and blocked in 2.5% horse serum for 20 min at room temperature. Subsequently, actin was stained with Ab176753 at 1/1000 dilution in 2.5% horse serum for 90 min at room temperature. Nuclear counterstaining with DAPI.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ross Beaumont

Verified customer

Submitted May 18 2021

lab Manager

 Good Good 4/5 (Ease of Use)
Abreviews
The reagent works
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 27 2019

CytoPainter Phalloidin-iFluor 488 labeling of plant material

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
Detection of F-actin in live pollen of 3 species: Gagea lutea, Saintpaulia ionatha and Yucca filamentosa:

Pollen from - 20 degrees Celsius were placed in 4 degrees Celsius for a night.
Next day placed in PBS buffer for 5 min and transferred into 1xPhalloidin-iFluor 488 working solution (0,5 ul + 500 ul PBS buffer) for 20 or 90 min at RT in darkness.
Immediately visualized using Leica DM6000B with FITC filter.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 16 2019

Phalloidin-iFluor 488 Reagent

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
Bronchial epithelial cell line. 4% paraformaldehyde fixation for 15min RT. Stain with Phalloidin (1:1000) for 45 min at RT and nucleus co-stained with DAPI.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 10 2019

Good staining

 Good Good 4/5 (Ease of Use)
Abreviews
abreview image
Epithelial cells:
- Fixed 4% PFA (room temperature)
- Dilution 1:1000
- DAPI co-staining
- Fluorescence measured with confocal microscopy
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 24 2019

Whole mount IF for the inner ear

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
I used abcam's phalloidin-488 for whole mount IF of mouse inner ear (sensory epithelia). It stains actin so stereocilia are stained very strong. Diluted 1:1000 in PBS and incubated for 2 hours in RT.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Shahar Taiber

Verified customer

Submitted Jun 04 2018

Phalloidin immunofluorescence of lim2099 cells

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
Seeding of 200.000 cells/well on cover slips in a 24-well-plate overnight.
5 min washing with PBS 0.1% Tween
20 min fixation with 4% PFA at room temperature.
3 x 5 min washing with PBS 0.1% Tween
10 min permeabilization with PBS 0.3% Triton-X 100
3 x 5 min washing with PBS 0.1% Tween.
Phalloidin staining for 1 hour at room temperature in the dark: 100 µl of 1x reagent diluted in PBS 0.1% Triton-X 100 1.5% BSA per coverslip.
3 x 5 min PBS 0.1% Tween.
Mounting of coverslips with ProLong Gold Antifade Mountant with DAPI on microscope slides.
Drying overnight at 4°C and imaging on the next day.

CytoPainter Phalloidin-iFluor 488 Reagent is an excellent stain yielding great results at low expenses in comparison to other more expensive stains from competing companies.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 29 2017

F-Actin / Peritoneal Macrophages

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
Excellent product for F-Actin staining. Very bright, yields minimal background if any when used correctly. Can be used for z-stacking without losing the signal in steps.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Gokhan Yilmaz

Verified customer

Submitted Mar 31 2017

1-10 of 13 Abreviews or Q&A

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