Phalloidin-iFluor 488 Reagent (ab176753)
Key features and details
- Assay type: Cell-based (qualitative)
- Platform: Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Phalloidin-iFluor 488 Reagent
See all F-actin kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Product overview
Phalloidin-iFluor 488 Reagent ab176753 is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. The iFluor 488 dye can be easily detected with a fluorescent microscope at Ex/Em = 493/517 nm.
Our phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used with paraffin-embedded samples that have been de-paraffinized.
Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 647, Phalloidin-iFluor 594, Phalloidin-iFluor 555, and Rhodamine Phalloidin, search the website to see all phallodin conjugates, or read the phalloidin staining protocol.
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Notes
Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.
When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 300 tests Phalloidin-iFluor 488 Conjugate 1 x 300 tests -
Research areas
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Alternative names
- actin filament
- f actin
- Filamentous actin
Images
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Whole Mount Immunofluorescence of Mouse Inner EarImage courtesy of Mr. Shahar Taiber
Ab176753 at 1/1000 dilution in PBS whole mount Immunofluorescence of Mouse inner ear sensory epithelia. Tissue was incubated for 2 hours at room temperature.
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Phalloidin iFluor 488 staining of macrophagesReview by Dr. Jose Ramos VivasMacrophage J774A.1 cell line stained with Phalloidin 488 and DAPI nuclear counterstaining.
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CytoPainter Phalloidin-iFluor 488 Reagent (ab176753)Actin filaments staining in HeLa cells. Actin filaments (green) were stained with CytoPainter Phalloidin-iFluor 488 reagent (ab176753); tubulin filaments were stained with a mouse anti-tubulin antibody/goat anti-mouse IgG (red). Nuclei were stained with Hoechst 33342.
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CytoPainter Phalloidin-iFluor 488 Reagent (ab176753)Excitation and emission spectra of phalloidin-iFluor 488 reagent.
Datasheets and documents
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SDS download
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Datasheet download
References (124)
ab176753 has been referenced in 124 publications.
- Dewan SMR et al. Complement C5a-triggered differentiated HL-60 stimulates migration of THP-1 monocytic leukocytes via secretion of CCL2. FEBS Open Bio N/A:N/A (2021). PubMed: 33715310
- Zhang J et al. Primary human colonic mucosal barrier crosstalk with super oxygen-sensitive Faecalibacterium prausnitzii in continuous culture. Med (N Y) 2:74-98.e9 (2021). PubMed: 33511375
- Rashed FB et al. Identification of proteins and cellular pathways targeted by 2-nitroimidazole hypoxic cytotoxins. Redox Biol 41:101905 (2021). PubMed: 33640700
- Nagamachi A et al. Multiorgan failure with abnormal receptor metabolism in mice mimicking Samd9/9L syndromes. J Clin Invest 131:N/A (2021). PubMed: 33373325
- Bagher M et al. Crosstalk between Mast Cells and Lung Fibroblasts Is Modified by Alveolar Extracellular Matrix and Influences Epithelial Migration. Int J Mol Sci 22:N/A (2021). PubMed: 33419174