Name: Phalloidin-iFluor 488 Reagent
Brand: Cytopainter
SKU: ab176753
Rating: 5 (7 reviews)

Product name

Phalloidin-iFluor 488 Reagent
See all F-actin kits
Sample type

Adherent cells, Suspension cells
Assay type

Cell-based (qualitative)
Product overview

Phalloidin-iFluor 488 Reagent ab176753 is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. The iFluor 488 dye can be easily detected with a fluorescent microscope at Ex/Em = 493/517 nm.

Our phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used with paraffin-embedded samples that have been de-paraffinized.

Review our other popular phalloidin dye conjugates, including Phalloidin-iFluor 647, Phalloidin-iFluor 594, Phalloidin-iFluor 555, and Rhodamine Phalloidin, or search our website to see the rest of the range.
Notes

Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.

When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.
Platform

Fluorescence microscope

Storage instructions

Store at -20°C. Please refer to protocols.
Components 300 tests

Phalloidin-iFluor 488 Conjugate 1 x 300 tests
Research areas

CytoPainter Phalloidin-iFluor 488 Reagent (ab176753)

Actin filaments staining in HeLa cells. Actin filaments (green) were stained with CytoPainter Phalloidin-iFluor 488 reagent (ab176753); tubulin filaments were stained with a mouse anti-tubulin antibody/goat anti-mouse IgG (red). Nuclei were stained with Hoechst 33342.
CytoPainter Phalloidin-iFluor 488 Reagent (ab176753)

Excitation and emission spectra of phalloidin-iFluor 488 reagent.

Protocol Booklet

This product has been referenced in:

Wang JN et al. Acetyl-macrocalin B suppresses tumor growth in esophageal squamous cell carcinoma and exhibits synergistic anti-cancer effects with the Chk1/2 inhibitor AZD7762. Toxicol Appl Pharmacol 365:71-83 (2019). Read more (PubMed: 30633885) »
Hacohen-Kleiman G et al. Atypical Auditory Brainstem Response and Protein Expression Aberrations Related to ASD and Hearing Loss in the Adnp Haploinsufficient Mouse Brain. Neurochem Res N/A:N/A (2019). Read more (PubMed: 30659505) »

See all 26 Publications for this product

Publishing research using ab176753? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Epithelial cells:
- Fixed 4% PFA (room temperature)
- Dilution 1:1000
- DAPI co-staining
- Fluorescence measured with confocal microscopy

Abcam user community

Verified customer

Submitted Apr 24 2019

I used abcam's phalloidin-488 for whole mount IF of mouse inner ear (sensory epithelia). It stains actin so stereocilia are stained very strong. Diluted 1:1000 in PBS and incubated for 2 hours in RT.

Mr. Shahar Taiber

Verified customer

Submitted Jun 04 2018

Seeding of 200.000 cells/well on cover slips in a 24-well-plate overnight.
5 min washing with PBS 0.1% Tween
20 min fixation with 4% PFA at room temperature.
3 x 5 min washing with PBS 0.1% Tween
10 min permeabilization with PBS 0.3% Triton-X 100
3 x 5 min washing with PBS 0.1% Tween.
Phalloidin staining for 1 hour at room temperature in the dark: 100 µl of 1x reagent diluted in PBS 0.1% Triton-X 100 1.5% BSA per coverslip.
3 x 5 min PBS 0.1% Tween.
Mounting of coverslips with ProLong Gold Antifade Mountant with DAPI on microscope slides.
Drying overnight at 4°C and imaging on the next day.

CytoPainter Phalloidin-iFluor 488 Reagent is an excellent stain yielding great results at low expenses in comparison to other more expensive stains from competing companies.

Abcam user community

Verified customer

Submitted Jun 29 2017

Excellent product for F-Actin staining. Very bright, yields minimal background if any when used correctly. Can be used for z-stacking without losing the signal in steps.

Gokhan Yilmaz

Verified customer

Submitted Mar 31 2017

For our experiments, the macrophage J774A.1 cell line was placed in 24-well tissue culture plates containing round glass coverslips. After 24h, cells were washed four times and fixed with cold paraformaldehyde (3.2% in PBS) for 20 min at room temperature. After being fixed, the cells were permeabilized with Triton X-100 (0.1% in PBS) for 5 min at room temperature and washed four times with PBS. CytoPainter Phalloidin-iFluor 488 (1:1000 dilution in 1%BSA-PBS), which binds polymerized F-actin, was used to identify actin filaments and fibers. Coverslips were mounted on glass slides with mounting medium containing DAPI.
Preparations were examined with a Nikon A1R confocal scanning laser microscope equipped with 405nm/450nm (DAPI) and 488nm/525nm (CytoPainter iFluor) lasers. Images were captured at random with a ×20 (A), ×40 (B) or ×60 (C, D) objectives, and processed using the NIS-Elements 3.2 software. Scale bars: A, 50µm B, 10 µm.

Dr. Jose Ramos Vivas

Verified customer

Submitted Jun 24 2016

I stained the murine osteocyte cells (MLO-Y4 cells line) with Phalloidin-iFluor 488, DAPI staining and sclerostin antibody labbeled with iFluor 550. I used this protocol :
Fixed cells with PFA 4% for 20 min at room temperature
Wash 3 times for 5 min with PBS
Incubate for 10 min at room temperature with PBS-Triton 0.1%
Wash 3 times for 5 min with PBS-Tween 0,1%
Blocked for 30 min at room temprature with PBS-BSA 1%
Incubate with primary antibody in PBS-BSA 1% overnight at 4°C (antibody against sclerostin in this case)
Wash 3 times for 5 min with PBS-Tween 0,1%
Incubate with secondary antibody in PBS-BSA1% for 1h at room temperature in the dark (antibody anti-goat in this case)
Wash 3 times for 5 min with PBS-Tween 0,1%
Incubate with Phalloidin 488 PBS-BSA 1% 60 min at room temperature in the dark
Wash 3 times for 5 min with PBS-Tween 0,1%
Staining with DAPI for 15 min at room temprature in the dark
Wash 3 times for 5 min with PBS-Tween 0,1%
Air dry and mounting with fluorescent mounting medium

Abcam user community

Verified customer

Submitted Mar 11 2016

Huh-7 cells: 30 min incubation including 5 min prior treatment with 0.1% Triton-X.

Dr. Teresa Otto

Verified customer

Submitted Jul 22 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
For licensing inquiries, please contact partnerships@abcam.com

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Phalloidin-iFluor 488 Reagent (ab176753)

Overview

Properties

Images

Protocols

Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As

Good staining

Whole mount IF for the inner ear

Phalloidin immunofluorescence of lim2099 cells

F-Actin / Peritoneal Macrophages

CytoPainter Phalloidin-iFluor 488

Confocal image of MLO-Y4 cells stained with Phalloidin-iFluor 488 (& nuclear stained with DAP & sclerostin antibody labbeled with iFluor 550)

Good performance