Overview

  • Product name
    Phalloidin-iFluor 532 Reagent
    See all F-actin kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based (qualitative)
  • Product overview

    Phalloidin-iFluor 532 Reagent (ab176755) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-iFluor 532 dye can be easily detected with a fluorescent microscope at Ex/Em = 542/558 nm.


    Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.


    Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the dissociation rate of actin subunits from filaments ends, essentially stabilizing actin filaments through the prevention of filament depolymerization.

  • Tested applications
    Suitable for: FM, ICC/IFmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab176755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Images

  • Excitation and emission spectra of phalloidin-iFluor 532 reagent

Protocols

References

ab176755 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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