Phalloidin-iFluor 555 Reagent (ab176756)
Key features and details
- Assay type: Cell-based (qualitative)
- Platform: Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Overview
-
Product name
Phalloidin-iFluor 555 Reagent
See all F-actin kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Product overview
Phalloidin-iFluor 555 Reagent (ab176756) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phallodin-iFluor 555 can be detected with a fluorescent microscope at Ex/Em = 556/574 nm.
Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.
Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 488, Phalloidin-iFluor 647, Phalloidin-iFluor 594, and Rhodamine Phalloidin, search the website to see all phallodin conjugates, or read the phalloidin staining protocol.
-
Notes
Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.
When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.
-
Platform
Fluorescence microscope
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 300 tests Phalloidin-iFluor 555 Conjugate 1 x 300 tests -
Research areas
-
Alternative names
- actin filament
- f actin
- Filamentous actin
Associated products
Images
-
Functional Studies - Phalloidin-iFluor 555 Reagent (ab176756)Wong, Michael K et al., PloS one?vol. 13,6 e0199632., Fig 6, doi:10.1371/journal.pone.0199632Immunofluorescent images of Phalloidin staining at day 3 across various surface thicknesses. Red fluorescence indicates phalloidin staining for F-actin and blue fluorescence indicates DAPI staining for cell nuclei.
-
CytoPainter Phalloidin-iFluor 555 Reagent (ab176756)Actin filaments staining in HeLa cells. Actin filaments (red) were stained with CytoPainter Phalloidin-iFluor 555 reagent (ab176755). Nuclei were stained with Nuclear Green DCS1 (ab138905).
-
CytoPainter Phalloidin-iFluor 555 Reagent (ab176756)Excitation and emission of phalloidin-iFluor 555 reagent.
Datasheets and documents
-
SDS download
-
Datasheet download
References (27)
ab176756 has been referenced in 27 publications.
- Uretmen Kagiali ZC et al. CLIC4 and CLIC1 bridge plasma membrane and cortical actin network for a successful cytokinesis. Life Sci Alliance 3:N/A (2020). PubMed: 31879279
- Einstein SA et al. Application of Trifluoroacetic Acid as a Theranostic Agent for Chemical Ablation of Solid Tissue. J Vasc Interv Radiol 31:169-175 (2020). PubMed: 31537410
- Tong X et al. Suppression of AMP-activated protein kinase reverses osteoprotegerin-induced inhibition of osteoclast differentiation by reducing autophagy. Cell Prolif 53:e12714 (2020). PubMed: 31696568
- Coazzoli M et al. Acid Sphingomyelinase Downregulation Enhances Mitochondrial Fusion and Promotes Oxidative Metabolism in a Mouse Model of Melanoma. Cells 9:N/A (2020). PubMed: 32244541
- Karagonlar ZF et al. A Novel Function for KLF4 in Modulating the De-differentiation of EpCAM-/CD133- nonStem Cells into EpCAM+/CD133+ Liver Cancer Stem Cells in HCC Cell Line HuH7. Cells 9:N/A (2020). PubMed: 32408542