Phalloidin-iFluor 555 Reagent (ab176756)
- Datasheet
- References (7)
- Protocols
Overview
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Product namePhalloidin-iFluor 555 Reagent
See all F-actin kits -
Sample typeAdherent cells, Suspension cells
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Assay typeCell-based (qualitative)
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Product overview
Phalloidin-iFluor 555 Reagent (ab176756) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phallodin-iFluor 555 can be detected with a fluorescent microscope at Ex/Em = 556/574 nm.
Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.
Review our other popular phalloidin dye conjugates, including Phalloidin-iFluor 488, Phalloidin-iFluor 647, Phalloidin-iFluor 594, and Rhodamine Phalloidin, or search our website to see the rest of the range.
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Notes
Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.
When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.
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Tested applicationsSuitable for: FM, ICC/IFmore details
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 300 tests Phalloidin-iFluor 555 Conjugate 1 x 300 tests -
Research areas
Applications
Our Abpromise guarantee covers the use of ab176756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| FM | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. |
Images
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CytoPainter Phalloidin-iFluor 555 Reagent (ab176756)Actin filaments staining in HeLa cells. Actin filaments (red) were stained with CytoPainter Phalloidin-iFluor 555 reagent (ab176755). Nuclei were stained with Nuclear Green DCS1 (ab138905).
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CytoPainter Phalloidin-iFluor 555 Reagent (ab176756)Excitation and emission of phalloidin-iFluor 555 reagent.
Datasheets and documents
Protocols
References
This product has been referenced in:
- Yang X et al. Cadherins in the retinal pigment epithelium (RPE) revisited: P-cadherin is the highly dominant cadherin expressed in human and mouse RPE in vivo. PLoS One 13:e0191279 (2018). Read more (PubMed: 29338041) »
- Chanda A et al. Electrospun chitosan/polycaprolactone-hyaluronic acid bilayered scaffold for potential wound healing applications. Int J Biol Macromol 116:774-785 (2018). ICC/IF . Read more (PubMed: 29777811) »
