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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin
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Phalloidin-iFluor 555 Reagent (ab176756)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (3) Submit a question References (27)

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Functional Studies - Phalloidin-iFluor 555 Reagent (ab176756)
  • CytoPainter Phalloidin-iFluor 555 Reagent (ab176756)
  • CytoPainter Phalloidin-iFluor 555 Reagent (ab176756)

Key features and details

  • Assay type: Cell-based (qualitative)
  • Platform: Fluorescence microscope
  • Sample type: Adherent cells, Suspension cells

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Overview

  • Product name

    Phalloidin-iFluor 555 Reagent
    See all F-actin kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based (qualitative)
  • Product overview

    Phalloidin-iFluor 555 Reagent (ab176756) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phallodin-iFluor 555 can be detected with a fluorescent microscope at Ex/Em = 556/574 nm.


    Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.


    Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 488, Phalloidin-iFluor 647, Phalloidin-iFluor 594, and Rhodamine Phalloidin, search the website to see all phallodin conjugates, or read the phalloidin staining protocol.

  • Notes

    Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.

    When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.

  • Platform

    Fluorescence microscope

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 300 tests
    Phalloidin-iFluor 555 Conjugate 1 x 300 tests
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Kits/ Lysates/ Other
    • Kits
    • Cell Staining Kits
    • F-actin Staining Kits
  • Alternative names

    • actin filament
    • f actin
    • Filamentous actin

Associated products

    Images

    • Functional Studies - Phalloidin-iFluor 555 Reagent (ab176756)
      Functional Studies - Phalloidin-iFluor 555 Reagent (ab176756)Wong, Michael K et al., PloS one?vol. 13,6 e0199632., Fig 6, doi:10.1371/journal.pone.0199632
      Immunofluorescent images of Phalloidin staining at day 3 across various surface thicknesses. Red fluorescence indicates phalloidin staining for F-actin and blue fluorescence indicates DAPI staining for cell nuclei.
    • CytoPainter Phalloidin-iFluor 555 Reagent (ab176756)
      CytoPainter Phalloidin-iFluor 555 Reagent (ab176756)

      Actin filaments staining in HeLa cells. Actin filaments (red) were stained with CytoPainter Phalloidin-iFluor 555 reagent (ab176755). Nuclei were stained with Nuclear Green DCS1 (ab138905).

    • CytoPainter Phalloidin-iFluor 555 Reagent (ab176756)
      CytoPainter Phalloidin-iFluor 555 Reagent (ab176756)

      Excitation and emission of phalloidin-iFluor 555 reagent.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (27)

    Publishing research using ab176756? Please let us know so that we can cite the reference in this datasheet.

    ab176756 has been referenced in 27 publications.

    • Uretmen Kagiali ZC  et al. CLIC4 and CLIC1 bridge plasma membrane and cortical actin network for a successful cytokinesis. Life Sci Alliance 3:N/A (2020). PubMed: 31879279
    • Einstein SA  et al. Application of Trifluoroacetic Acid as a Theranostic Agent for Chemical Ablation of Solid Tissue. J Vasc Interv Radiol 31:169-175 (2020). PubMed: 31537410
    • Tong X  et al. Suppression of AMP-activated protein kinase reverses osteoprotegerin-induced inhibition of osteoclast differentiation by reducing autophagy. Cell Prolif 53:e12714 (2020). PubMed: 31696568
    • Coazzoli M  et al. Acid Sphingomyelinase Downregulation Enhances Mitochondrial Fusion and Promotes Oxidative Metabolism in a Mouse Model of Melanoma. Cells 9:N/A (2020). PubMed: 32244541
    • Karagonlar ZF  et al. A Novel Function for KLF4 in Modulating the De-differentiation of EpCAM-/CD133- nonStem Cells into EpCAM+/CD133+ Liver Cancer Stem Cells in HCC Cell Line HuH7. Cells 9:N/A (2020). PubMed: 32408542
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Excellent staining used for confocal and SIM

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    This product worked well for staining primary mouse BMDMs infected with Burkholderia, which hijacks the actin cytoskeleton. Stained cells at 1:1000-1:2000 according to protocol with good success. Images collected by confocal and SIM.

    Dr. David Place

    Verified customer

    Submitted Aug 18 2020

    Phalloidin iFluor 555 staining

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    Clear staining of actin stress fibers were observed in cultured swine testicle cells. Cells were fixed with 3% paraformaldehyde for 10 minutes and permeabilized for 5 minutes with 0.1% Triton X 100. Cells were stained with Phalloidin iFluor 555 for 1 hour at 37°C and counterstained with Hoechst 33342.

    Abcam user community

    Verified customer

    Submitted Dec 14 2019

    Phalloidin staining of paraffin embeded tissue.

    Poor Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    I purchased Phalloidin to stain paraffin embedded tissue, but it does not work. I tried many variations of time and temperature, concentration, with or without antigen retrieval, permeabilization and there was not a trace of fluorescent staining. Although as my positive control I used formalin fixed primary hepatocytes on chamber slides and that was very good.

    Abcam user community

    Verified customer

    Submitted Aug 03 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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