Overview

  • Product name
    Phalloidin-iFluor 555 Reagent
    See all F-actin kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based (qualitative)
  • Product overview

    Phalloidin-iFluor 555 Reagent (ab176756) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phallodin-iFluor 555 can be detected with a fluorescent microscope at Ex/Em = 556/574 nm.


    Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.


    Review our other popular phalloidin dye conjugates, including Phalloidin-iFluor 488Phalloidin-iFluor 647Phalloidin-iFluor 594, and Rhodamine Phalloidin, or search our website to see the rest of the range.

  • Notes

    Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.

    When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.

  • Platform
    Fluorescence microscope

Properties

Images

  • Immunofluorescent images of Phalloidin staining at day 3 across various surface thicknesses. Red fluorescence indicates phalloidin staining for F-actin and blue fluorescence indicates DAPI staining for cell nuclei.
  • Actin filaments staining in HeLa cells. Actin filaments (red) were stained with CytoPainter Phalloidin-iFluor 555 reagent (ab176755). Nuclei were stained with Nuclear Green DCS1 (ab138905).

  • Excitation and emission of phalloidin-iFluor 555 reagent.

Protocols

References

This product has been referenced in:
  • Pilling D  et al. Different Isoforms of the Neuronal Guidance Molecule Slit2 Directly Cause Chemoattraction or Chemorepulsion of Human Neutrophils. J Immunol 202:239-248 (2019). Read more (PubMed: 30510066) »
  • Lahrouchi N  et al. Homozygous frameshift mutations in FAT1 cause a syndrome characterized by colobomatous-microphthalmia, ptosis, nephropathy and syndactyly. Nat Commun 10:1180 (2019). Read more (PubMed: 30862798) »
See all 11 Publications for this product

Customer reviews and Q&As

Phalloidin staining of paraffin embeded tissue.

Poor Excellent 5/5 (Ease of Use)
Abreviews
I purchased Phalloidin to stain paraffin embedded tissue, but it does not work. I tried many variations of time and temperature, concentration, with or without antigen retrieval, permeabilization and there was not a trace of fluorescent staining. Although as my positive control I used formalin fixed primary hepatocytes on chamber slides and that was very good.

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Verified customer

Submitted Aug 03 2017

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