Phalloidin-iFluor 555 Reagent (ab176756)
- Datasheet
- References (11)
- Protocols
Overview
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Product namePhalloidin-iFluor 555 Reagent
See all F-actin kits -
Sample typeAdherent cells, Suspension cells
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Assay typeCell-based (qualitative)
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Product overview
Phalloidin-iFluor 555 Reagent (ab176756) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phallodin-iFluor 555 can be detected with a fluorescent microscope at Ex/Em = 556/574 nm.
Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.
Review our other popular phalloidin dye conjugates, including Phalloidin-iFluor 488, Phalloidin-iFluor 647, Phalloidin-iFluor 594, and Rhodamine Phalloidin, or search our website to see the rest of the range.
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Notes
Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.
When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.
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PlatformFluorescence microscope
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 300 tests Phalloidin-iFluor 555 Conjugate 1 x 300 tests -
Research areas
Images
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Functional Studies - Phalloidin-iFluor 555 Reagent (ab176756)Wong, Michael K et al., PloS one?vol. 13,6 e0199632., Fig 6, doi:10.1371/journal.pone.0199632Immunofluorescent images of Phalloidin staining at day 3 across various surface thicknesses. Red fluorescence indicates phalloidin staining for F-actin and blue fluorescence indicates DAPI staining for cell nuclei. -
CytoPainter Phalloidin-iFluor 555 Reagent (ab176756)Actin filaments staining in HeLa cells. Actin filaments (red) were stained with CytoPainter Phalloidin-iFluor 555 reagent (ab176755). Nuclei were stained with Nuclear Green DCS1 (ab138905).
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CytoPainter Phalloidin-iFluor 555 Reagent (ab176756)Excitation and emission of phalloidin-iFluor 555 reagent.
Protocols
References
This product has been referenced in:
- Pilling D et al. Different Isoforms of the Neuronal Guidance Molecule Slit2 Directly Cause Chemoattraction or Chemorepulsion of Human Neutrophils. J Immunol 202:239-248 (2019). Read more (PubMed: 30510066) »
- Lahrouchi N et al. Homozygous frameshift mutations in FAT1 cause a syndrome characterized by colobomatous-microphthalmia, ptosis, nephropathy and syndactyly. Nat Commun 10:1180 (2019). Read more (PubMed: 30862798) »
