• Product name
    Phalloidin-iFluor 594 Reagent
    See all F-actin kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based (qualitative)
  • Product overview

    Phalloidin-iFluor 594 Reagent (ab176757) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-Fluor 594 can be detected with a fluorescent microscope at Ex/Em = 590/618 nm.

    Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.

    Review our other popular phalloidin dye conjugates, including Phalloidin-iFluor 488Phalloidin-iFluor 647Phalloidin-iFluor 555, and Rhodamine Phalloidin, or search our website to see the rest of the range.

  • Notes

    Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.

    When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.

  • Platform
    Fluorescence microscope



  • The A549 KOAGR2 were stained with CytoPainter phalloidin-iFluor 594 reagent to detect morphological changes associated with F-actin reorganization. Nuclei were visualised with DAPI
  • Actin filaments staining in HeLa cells. Actin filaments (red) were stained with CytoPainter Phalloidin-iFluor 594 reagent (ab176757); ER (green) was stained with CytoPainter ER Staining Kit – Green Fluorescence (ab139481). Nuclei (blue) were stained with DAPI.

  • Excitation and emission spectra of phalloidin-iFluor 594 reagent.



This product has been referenced in:
  • Lorén CE  et al. Low Molecular Mass Myocardial Hyaluronan in Human Hypertrophic Cardiomyopathy. Cells 8:N/A (2019). Read more (PubMed: 30699940) »
  • Kami D  et al. The DEAD-box RNA-binding protein DDX6 regulates parental RNA decay for cellular reprogramming to pluripotency. PLoS One 13:e0203708 (2018). Read more (PubMed: 30273347) »
See all 8 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Whole mount IF for the inner ear

Excellent Excellent 5/5 (Ease of Use)
I used abcam's phalloidin-594 for a whole-mount of the mouse inner ear (sensory epithelium). Diluted 1:1000 in PBS, incubated at RT for 2 hours. very strong staining, needed to really cut down on the laser.

Mr. Shahar Taiber

Verified customer

Submitted Jun 04 2018

Mouse kidneys were perfused in through cardiac puncture with 0.9% NaCl. Kidneys were fixed with formalin overnight before paraffin embedding and sectioning. Deparaffinization & Rehydration was performed exactly as per Abcam protocols. Permeabilisation was performed using 0.1% Triton-X100 in PBS for 10 min at room temperature. Heat-induced epitope retrieval was performed by incubation in Tris-EDTA buffer for 20 min inside of a boiling, vegetable steamer. Blocking was performed using 5% BSA in TBS for 2 hours at room temperature. Phalloidin-iFluor 594 Reagent (ab176757) was diluted at the concentration 1:100 in a solution of 1% BSA in TBS for 1h at room temperature protected from light.

Guy Cameron

Verified customer

Submitted Jan 13 2018

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