Product namePhalloidin-iFluor 594 Reagent
See all F-actin kits
Sample typeAdherent cells, Suspension cells
Assay typeCell-based (qualitative)
Phalloidin-iFluor 594 Reagent (ab176757) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-Fluor 594 can be detected with a fluorescent microscope at Ex/Em = 590/618 nm.
Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.
Review our other popular phalloidin dye conjugates, including Phalloidin-iFluor 488, Phalloidin-iFluor 647, Phalloidin-iFluor 555, and Rhodamine Phalloidin, or search our website to see the rest of the range.
Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.
When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 300 tests Phalloidin-iFluor 594 Conjugate 1 x 300 tests
The A549 KOAGR2 were stained with CytoPainter phalloidin-iFluor 594 reagent to detect morphological changes associated with F-actin reorganization. Nuclei were visualised with DAPI
Actin filaments staining in HeLa cells. Actin filaments (red) were stained with CytoPainter Phalloidin-iFluor 594 reagent (ab176757); ER (green) was stained with CytoPainter ER Staining Kit – Green Fluorescence (ab139481). Nuclei (blue) were stained with DAPI.
Excitation and emission spectra of phalloidin-iFluor 594 reagent.
This product has been referenced in:
- Lorén CE et al. Low Molecular Mass Myocardial Hyaluronan in Human Hypertrophic Cardiomyopathy. Cells 8:N/A (2019). Read more (PubMed: 30699940) »
- Kami D et al. The DEAD-box RNA-binding protein DDX6 regulates parental RNA decay for cellular reprogramming to pluripotency. PLoS One 13:e0203708 (2018). Read more (PubMed: 30273347) »