Phalloidin-iFluor 633 Reagent (ab176758)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Phalloidin-iFluor 633 Reagent
See all F-actin kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Product overview
Phalloidin-iFluor 633 Reagent (ab176758) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-iFluor 633 can be detected with a fluorescent microscope at Ex/Em = 634/649 nm.
Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.
Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 488, Phalloidin-iFluor 647, Phalloidin-iFluor 594, Phalloidin-iFluor 555, and Rhodamine Phalloidin, search the website to see all phallodin conjugates, or read the phalloidin staining protocol.
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Notes
Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.
When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 300 tests Phalloidin-iFluor 633 Conjugate 300 tests -
Research areas
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Alternative names
- actin filament
- f actin
- Filamentous actin
Images
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CytoPainter Phalloidin-iFluor 633 Reagent (ab176758)Excitation and emission spectra of phalloidin-iFluor 633 reagent.
Protocols
References
This product has been referenced in:
- Tsui TY et al. What's Happening on the Other Side? Revealing Nano-Meter Scale Features of Mammalian Cells on Engineered Textured Tantalum Surfaces. Materials (Basel) 12:N/A (2018). Read more (PubMed: 30602684) »
- Nagai T & Mizuno K Jasplakinolide induces primary cilium formation through cell rounding and YAP inactivation. PLoS One 12:e0183030 (2017). Read more (PubMed: 28797107) »
