Key features and details
- Assay type: Cell-based (qualitative)
- Platform: Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Product namePhalloidin-iFluor 633 Reagent
See all F-actin kits
Sample typeAdherent cells, Suspension cells
Assay typeCell-based (qualitative)
Phalloidin-iFluor 633 Reagent (ab176758) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-iFluor 633 can be detected with a fluorescent microscope at Ex/Em = 634/649 nm.
Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.
Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 488, Phalloidin-iFluor 647, Phalloidin-iFluor 594, Phalloidin-iFluor 555, and Rhodamine Phalloidin, search the website to see all phallodin conjugates, or read the phalloidin staining protocol.
Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.
When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 300 tests Phalloidin-iFluor 633 Conjugate 1 x 300 tests
- actin filament
- f actin
- Filamentous actin
ab176758 has been referenced in 4 publications.
- Baker LA et al. Proteogenomic analysis of Inhibitor of Differentiation 4 (ID4) in basal-like breast cancer. Breast Cancer Res 22:63 (2020). PubMed: 32527287
- Koundouros N et al. Metabolic Fingerprinting Links Oncogenic PIK3CA with Enhanced Arachidonic Acid-Derived Eicosanoids. Cell 181:1596-1611.e27 (2020). PubMed: 32559461
- Tsui TY et al. What's Happening on the Other Side? Revealing Nano-Meter Scale Features of Mammalian Cells on Engineered Textured Tantalum Surfaces. Materials (Basel) 12:N/A (2018). PubMed: 30602684
- Nagai T & Mizuno K Jasplakinolide induces primary cilium formation through cell rounding and YAP inactivation. PLoS One 12:e0183030 (2017). PubMed: 28797107