Overview

  • Product name
    Phalloidin-iFluor 680 Reagent
    See all F-actin kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based (qualitative)
  • Product overview

    Phalloidin-iFluor 680 Reagent (ab176760) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phallodin-iFluor 680 can be detected with a fluorescent microscope at Ex/Em = 650/665 nm (NIR range).


    Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.


    Review our other popular phalloidin dye conjugates, including Phalloidin-iFluor 488Phalloidin-iFluor 647Phalloidin-iFluor 594Phalloidin-iFluor 555, and Rhodamine Phalloidin, or search our website to see the rest of the range.


     

  • Notes

    Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.

    When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.

  • Platform
    Fluorescence microscope

Properties

Images

  • Actin filaments staining in HeLa cells. Actin filaments (red) were stained with CytoPainter Phalloidin-iFluor 680 reagent (ab176760). Nuclei were stained with Nuclear Green DCS1 (ab138905).

  • Excitation and emission spectra of phalloidin-iFluor 680 reagent.

Protocols

References

ab176760 has not yet been referenced specifically in any publications.

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