Overview

  • Product name
    Phalloidin-iFluor 750 Reagent
    See all F-actin kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based (qualitative)
  • Product overview

    Phalloidin-iFluor 750 Reagent (ab176762) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-iFluor 750 can be detected with a fluorescent microscope at Ex/Em = 752/778 nm (NIR range).


    phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.


    Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the dissociation rate of actin subunits from filaments ends, essentially stabilizing actin filaments through the prevention of filament depolymerization.

  • Platform
    Fluorescence microscope

Properties

Images

  • Excitation and emission spectra of phalloidin-iFluor 750 reagent.

Protocols

References

ab176762 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

The vial of ab176762 is lyophylized solid, so simply add 30 uL of DMSO into the vial to make a 1000X stock solution. The assay is based on 100 uL/well/assay with 1X solution.

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