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    phalloidin-ifluor-750-reagent-ab176762.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin
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Phalloidin-iFluor 750 Reagent (ab176762)

  • Datasheet
  • SDS
  • Protocol Booklet
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CytoPainter Phalloidin-iFluor 750 Reagent (ab176762)

    Key features and details

    • Assay type: Cell-based (qualitative)
    • Platform: Fluorescence microscope
    • Sample type: Adherent cells, Suspension cells

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    Overview

    • Product name

      Phalloidin-iFluor 750 Reagent
      See all F-actin kits
    • Sample type

      Adherent cells, Suspension cells
    • Assay type

      Cell-based (qualitative)
    • Product overview

      Phalloidin-iFluor 750 Reagent (ab176762) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-iFluor 750 can be detected with a fluorescent microscope at Ex/Em = 752/778 nm (NIR range).


      phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.


      Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the dissociation rate of actin subunits from filaments ends, essentially stabilizing actin filaments through the prevention of filament depolymerization.

    • Platform

      Fluorescence microscope

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 300 tests
      Phalloidin-iFluor 750 Conjugate 1 x 300 tests
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Actin
      • Kits/ Lysates/ Other
      • Kits
      • Cell Staining Kits
      • F-actin Staining Kits
    • Alternative names

      • actin filament
      • f actin
      • Filamentous actin

    Images

    • CytoPainter Phalloidin-iFluor 750 Reagent (ab176762)
      CytoPainter Phalloidin-iFluor 750 Reagent (ab176762)

      Excitation and emission spectra of phalloidin-iFluor 750 reagent.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab176762? Please let us know so that we can cite the reference in this datasheet.

    ab176762 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    How much product is provided?

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    Abcam community

    Verified customer

    Asked on Jan 30 2014

    Answer

    The vial of ab176762 is lyophylized solid, so simply add 30 uL of DMSO into the vial to make a 1000X stock solution. The assay is based on 100 uL/well/assay with 1X solution.

    Read More

    Ariana Veiga

    Abcam Scientific Support

    Answered on Jan 30 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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