Phalloidin-iFluor 750 Reagent (ab176762)

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Overview

  • Product name

    Phalloidin-iFluor 750 Reagent
    See all F-actin kits

  • Sample type

    Adherent cells, Suspension cells

  • Assay type

    Cell-based (qualitative)

  • Product overview

    Phalloidin-iFluor 750 Reagent (ab176762) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-iFluor 750 can be detected with a fluorescent microscope at Ex/Em = 752/778 nm (NIR range).


    phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.


    Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the dissociation rate of actin subunits from filaments ends, essentially stabilizing actin filaments through the prevention of filament depolymerization.

  • Platform

    Fluorescence microscope

Properties

Images

  • CytoPainter Phalloidin-iFluor 750 Reagent (ab176762)
    CytoPainter Phalloidin-iFluor 750 Reagent (ab176762)

    Excitation and emission spectra of phalloidin-iFluor 750 reagent.

Protocols

References

ab176762 has not yet been referenced specifically in any publications.

Publishing research using ab176762? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

How much product is provided?

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Answer

The vial of ab176762 is lyophylized solid, so simply add 30 uL of DMSO into the vial to make a 1000X stock solution. The assay is based on 100 uL/well/assay with 1X solution.

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