Overview

  • Product name
  • Description
    Rabbit polyclonal to PHAP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 149-163 of Human I1 alpha PP2A.

Properties

Applications

Our Abpromise guarantee covers the use of ab1181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
Reactive with I1aPP2A from diverse tissue sources by Western blotting. Used at 1/100 with cell extracts.

Target

  • Function
    Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.
  • Tissue specificity
    Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala.
  • Sequence similarities
    Belongs to the ANP32 family.
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
  • Post-translational
    modifications
    Phosphorylated on serine residues.
    The N-terminus is blocked.
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  • Cellular localization
    Nucleus. Cytoplasm. Endoplasmic reticulum. Translocates to the cytoplasm during the process of neuritogenesis (By similarity). Shuttles between nucleus and cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • acidic (leucine-rich) nuclear phosphoprotein 32 family, member A antibody
    • Acidic leucine-rich nuclear phosphoprotein 32 family member A antibody
    • Acidic nuclear phosphoprotein 32 family member A antibody
    • Acidic nuclear phosphoprotein pp32 antibody
    • AN32A_HUMAN antibody
    • ANP32A antibody
    • C15orf1 antibody
    • Cerebellar leucine rich acidic nuclear protein antibody
    • Hepatopoietin Cn antibody
    • HPPCn antibody
    • I1PP2A antibody
    • Inhibitor 1 of protein phosphatase 2A antibody
    • inhibitor-1 of protein phosphatase-2A antibody
    • Lanp antibody
    • Leucine rich acidic nuclear protein antibody
    • Leucine-rich acidic nuclear protein antibody
    • MAPM antibody
    • Mapmodulin antibody
    • MGC119787 antibody
    • MGC150373 antibody
    • PHAPI antibody
    • Potent heat stable protein phosphatase 2A inhibitor I1PP2A antibody
    • Potent heat-stable protein phosphatase 2A inhibitor I1PP2A antibody
    • PP32 antibody
    • Putative HLA DR associated protein I antibody
    • Putative HLA-DR-associated protein I antibody
    • Putative human HLA class II associated protein I antibody
    • Putative human HLA class II-associated protein antibody
    see all

Images

  • Western blot of 10 µg HEK 293 cell extracts with Anti- I1α (GLO149).

    Protocols

References

ab1181 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

I have confirmed with the laboratory that the conditions you have used to prepare the cell lysates seem appropriate and similar to what we use and the blocking agent seems to be appropriate. The difference we observe in the protocols is the incubation time and I therefore would like to recommend an overnight incubation (at 4C). If you still experience problems following this change please let me know and I will arrange a replacement vial as you purchased the antibody in the last 90days,

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Answer

Thank you for contacting us for technical support, I'm sorry to hear you are experiencing problems with ab1181. Could you please clarify a few more details so I can understand your problem better and resolve it? 1) what was the sample preparation: lysis buffer used (protease inhibitors?), lysis extraction time, did you boil the sample in reducing loading buffer? 2)how long did you incubate the primary antibody for? what buffer did you dilute it in? 3) what was the blocking reagent used? For how long? I looked at any possible shipping delays which may suggest a damaged antibody but did not find any problems, the parcel took under a day to reach you. Thank you in advance for providing those further details, I look forward to hearing from you,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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