Key features and details
- Rabbit polyclonal to PHAP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PHAP1 antibody
See all PHAP1 primary antibodies
DescriptionRabbit polyclonal to PHAP1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Xenopus laevis, Zebrafish, Xenopus tropicalis
Recombinant fragment, corresponding to a region within amino acids 1-178 of Human PHAP1 (UniProt P39687).
- 293T, A431, Jurkat, Raji and mouse brain lysates; A431 cells; Human BT474 xenograft tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 28 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).|
|ICC/IF||1/100 - 1/1000.|
FunctionImplicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.
Tissue specificityExpressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala.
Sequence similaritiesBelongs to the ANP32 family.
Contains 4 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
modificationsPhosphorylated on serine residues.
The N-terminus is blocked.
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
Cellular localizationNucleus. Cytoplasm. Endoplasmic reticulum. Translocates to the cytoplasm during the process of neuritogenesis (By similarity). Shuttles between nucleus and cytoplasm.
- Information by UniProt
- acidic (leucine-rich) nuclear phosphoprotein 32 family, member A antibody
- Acidic leucine-rich nuclear phosphoprotein 32 family member A antibody
- Acidic nuclear phosphoprotein 32 family member A antibody
Anti-PHAP1 antibody (ab155148) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 28 kDa
12% SDS PAGE
Anti-PHAP1 antibody (ab155148) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 28 kDa
12% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human BT474 xenograft tissue labeling PHAP1 with ab155148 at 1/100 dilution.
Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling PHAP1 with ab155148 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
ab155148 has not yet been referenced specifically in any publications.