- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameAnti-PHAP1 antibody
See all PHAP1 primary antibodies -
DescriptionRabbit polyclonal to PHAP1
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Host speciesRabbit
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SpecificityThis antibody does not cross react with PHAP12a or PHAPIII isoforms.
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Tested applicationsSuitable for: WB, ICC/IF, Blocking, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide (Human).
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Positive control
- Raji whole cell lysate.
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General notesStable for one year.
Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-mediated cell transformation.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Primary antibody notesApoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-mediated cell transformation.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab5991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa.Can be blocked with PHAP1 peptide (ab6240). | |
| ICC/IF | Use a concentration of 20 µg/ml. | |
| Blocking | Use at an assay dependent concentration. PubMed: 16489023 | |
| IHC-P | Use a concentration of 2 µg/ml. |
Target
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FunctionImplicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.
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Tissue specificityExpressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala.
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Sequence similaritiesBelongs to the ANP32 family.
Contains 4 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain. -
Post-translational
modificationsPhosphorylated on serine residues.
The N-terminus is blocked.
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. -
Cellular localizationNucleus. Cytoplasm. Endoplasmic reticulum. Translocates to the cytoplasm during the process of neuritogenesis (By similarity). Shuttles between nucleus and cytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 8125 Human
- Entrez Gene: 11737 Mouse
- Entrez Gene: 25379 Rat
- Omim: 600832 Human
- SwissProt: P39687 Human
- SwissProt: O35381 Mouse
- SwissProt: P49911 Rat
- Unigene: 458747 Human
see all -
Alternative names
- acidic (leucine-rich) nuclear phosphoprotein 32 family, member A antibody
- Acidic leucine-rich nuclear phosphoprotein 32 family member A antibody
- Acidic nuclear phosphoprotein 32 family member A antibody
see all
Images
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Western blot - Anti-PHAP1 antibody (ab5991)Western blot analysis of PHAP I expression in human Raji cell (A), mouse (B) and rat (C) testis tissue llysates with anti-PHAP I at 1µ g/ml. Western blot analysis of PHAP I expression in human Raji cell (A), mouse (B) and rat (C) testis tissue llysates with anti-PHAP I at 1 µg/ml -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHAP1 antibody (ab5991)ab5991 at 2µg/ml staining PHAP1 in mouse small intestine tissue by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5991)Immunofluorescence of PHAP I in Mouse Small Intestine cells using ab5991 at 20 ug/ml.
Protocols
References
This product has been referenced in:
- Saavedra F et al. PP32 and SET/TAF-Iß proteins regulate the acetylation of newly synthesized histone H4. Nucleic Acids Res 45:11700-11710 (2017). Read more (PubMed: 28977641) »
- Wang S et al. The expression and distributions of ANP32A in the developing brain. Biomed Res Int 2015:207347 (2015). Read more (PubMed: 25866766) »
Customer reviews and Q&As
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