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    phap1-antibody-ab5991.pdf

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Cell Biology Apoptosis Intracellular Caspases etc Caspases
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Anti-PHAP1 antibody (ab5991)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (4)

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Western blot - Anti-PHAP1 antibody (ab5991)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHAP1 antibody (ab5991)
  • Western blot - Anti-PHAP1 antibody (ab5991)
  • Western blot - Anti-PHAP1 antibody (ab5991)
  • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5991)

Key features and details

  • Rabbit polyclonal to PHAP1
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human PHAP1 protein (ab101155)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-PHAP1 antibody
    See all PHAP1 primary antibodies
  • Description

    Rabbit polyclonal to PHAP1
  • Host species

    Rabbit
  • Specificity

    This antibody does not cross react with PHAP12a or PHAPIII isoforms.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IHC-P
    Mouse
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human PHAP1 aa 199-249 (C terminal). The exact sequence is proprietary. A peptide corresponding to 14 amino acids near the carboxy terminus of human PHAP1.
    (Peptide available as ab6240)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-mediated cell transformation.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human PHAP1 protein (ab101155)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Mouse
IHC-P
Mouse
WB
Mouse
Human
Application Abreviews Notes
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa.
ICC/IF
Use a concentration of 20 µg/ml.
IHC-P
Use a concentration of 2 µg/ml.
Notes
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa.
ICC/IF
Use a concentration of 20 µg/ml.
IHC-P
Use a concentration of 2 µg/ml.

Target

  • Function

    Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.
  • Tissue specificity

    Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala.
  • Sequence similarities

    Belongs to the ANP32 family.
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
  • Post-translational
    modifications

    Phosphorylated on serine residues.
    The N-terminus is blocked.
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  • Cellular localization

    Nucleus. Cytoplasm. Endoplasmic reticulum. Translocates to the cytoplasm during the process of neuritogenesis (By similarity). Shuttles between nucleus and cytoplasm.
  • Target information above from: UniProt accession P39687 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8125 Human
    • Entrez Gene: 11737 Mouse
    • Entrez Gene: 25379 Rat
    • Omim: 600832 Human
    • SwissProt: P39687 Human
    • SwissProt: O35381 Mouse
    • SwissProt: P49911 Rat
    • Unigene: 458747 Human
    • Unigene: 269088 Mouse
    • Unigene: 10123 Rat
    see all
  • Alternative names

    • acidic (leucine-rich) nuclear phosphoprotein 32 family, member A antibody
    • Acidic leucine-rich nuclear phosphoprotein 32 family member A antibody
    • Acidic nuclear phosphoprotein 32 family member A antibody
    • Acidic nuclear phosphoprotein pp32 antibody
    • AN32A_HUMAN antibody
    • ANP32A antibody
    • C15orf1 antibody
    • Cerebellar leucine rich acidic nuclear protein antibody
    • Hepatopoietin Cn antibody
    • HPPCn antibody
    • I1PP2A antibody
    • Inhibitor 1 of protein phosphatase 2A antibody
    • inhibitor-1 of protein phosphatase-2A antibody
    • Lanp antibody
    • Leucine rich acidic nuclear protein antibody
    • Leucine-rich acidic nuclear protein antibody
    • MAPM antibody
    • Mapmodulin antibody
    • MGC119787 antibody
    • MGC150373 antibody
    • PHAPI antibody
    • Potent heat stable protein phosphatase 2A inhibitor I1PP2A antibody
    • Potent heat-stable protein phosphatase 2A inhibitor I1PP2A antibody
    • PP32 antibody
    • Putative HLA DR associated protein I antibody
    • Putative HLA-DR-associated protein I antibody
    • Putative human HLA class II associated protein I antibody
    • Putative human HLA class II-associated protein antibody
    see all

Images

  • Western blot - Anti-PHAP1 antibody (ab5991)
    Western blot - Anti-PHAP1 antibody (ab5991)
    All lanes : Anti-PHAP1 antibody (ab5991) at 1 µg/ml

    Lane 1 : 293 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : CaCo-2 cell lysate
    Lane 5 : Daudi cell lysate
    Lane 6 : HeLa cell lysate
    Lane 7 : HepG2 cell lysate
    Lane 8 : K562 cell lysate
    Lane 9 : MCF-7 cell lysate
    Lane 10 : Jurkat cell lysate
    Lane 11 : SK-N-SH cell lysate
    Lane 12 : THP-1 cell lysate
    Lane 13 : NIH/3T3 cell lysate
    Lane 14 : YB2/0 cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution


    Incubated with the primary antibody for 1 hour at room temperature in 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHAP1 antibody (ab5991)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHAP1 antibody (ab5991)

    ab5991 at 2µg/ml staining PHAP1 in mouse small intestine tissue by IHC

  • Western blot - Anti-PHAP1 antibody (ab5991)
    Western blot - Anti-PHAP1 antibody (ab5991)
    All lanes : Anti-PHAP1 antibody (ab5991) at 1 µg/ml

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Mouse pancreas tissue lysate
    Lane 3 : Mouse testis tissue lysate
    Lane 4 : Mouse liver tissue lysate
    Lane 5 : Mouse spleen tissue lysate
    Lane 6 : Mouse kidney tissue lysate
    Lane 7 : Mouse colon tissue lysate
    Lane 8 : Mouse stomach tissue lysate
    Lane 9 : Mouse brain tissue lysate
    Lane 10 : Mouse skin tissue lysate
    Lane 11 : Mouse skeletal muscle tissue lysate
    Lane 12 : Mouse bladder tissue lysate
    Lane 13 : Mouse lung tissue lysate
    Lane 14 : K562 cell lysate

    Lysates/proteins at 15 µg/ml per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution


    Incubated with the primary antibody for 1 hour at room temperature in 5% NFDM/TBST.

  • Western blot - Anti-PHAP1 antibody (ab5991)
    Western blot - Anti-PHAP1 antibody (ab5991)

    Western blot analysis of PHAP I expression in human Raji cell (A), mouse (B) and rat (C) testis tissue llysates with anti-PHAP I at 1 µg/ml.

  • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5991)
    Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5991)

    Immunofluorescence of PHAP I in mouse small intestine cells using ab5991 at 20µg/ml.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (4)

Publishing research using ab5991? Please let us know so that we can cite the reference in this datasheet.

ab5991 has been referenced in 4 publications.

  • Saavedra F  et al. PP32 and SET/TAF-Iß proteins regulate the acetylation of newly synthesized histone H4. Nucleic Acids Res 45:11700-11710 (2017). PubMed: 28977641
  • Wang S  et al. The expression and distributions of ANP32A in the developing brain. Biomed Res Int 2015:207347 (2015). PubMed: 25866766
  • Schafer ZT  et al. Enhanced sensitivity to cytochrome c-induced apoptosis mediated by PHAPI in breast cancer cells. Cancer Res 66:2210-8 (2006). WB, Blocking ; Human . PubMed: 16489023
  • Dirksen EH  et al. Human lymphoblastoid proteome analysis reveals a role for the inhibitor of acetyltransferases complex in DNA double-strand break response. Cancer Res 66:1473-80 (2006). WB ; Human . PubMed: 16452203

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Is the immunogen sequence available?

Read More

Abcam community

Verified customer

Asked on Jan 27 2005

Answer

The synthetic peptide corresponds to amino acids at carboxy terminus of human PHAP I. Unfortunately, the sequence is not available.

Read More

Abcam Scientific Support

Answered on Jan 27 2005

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