Anti-PHAP1 antibody (ab5992)
Key features and details
- Rabbit polyclonal to PHAP1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PHAP1 antibody
See all PHAP1 primary antibodies -
Description
Rabbit polyclonal to PHAP1 -
Host species
Rabbit -
Specificity
This antibody does not cross-react with PHAP12a or PHAPIII. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human PHAP1.
(Peptide available asab6241) -
Positive control
- WB: Daudi, HeLa, HepG2, K562 and Raji cell lysates. ICC/IF: Raji cells
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General notes
Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-mediated cell transformation.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-mediated cell transformation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Human
|
| All applications |
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 2 µg/ml.
|
|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 2 µg/ml. |
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa. |
Target
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Function
Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression. -
Tissue specificity
Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala. -
Sequence similarities
Belongs to the ANP32 family.
Contains 4 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain. -
Post-translational
modificationsPhosphorylated on serine residues.
The N-terminus is blocked.
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. -
Cellular localization
Nucleus. Cytoplasm. Endoplasmic reticulum. Translocates to the cytoplasm during the process of neuritogenesis (By similarity). Shuttles between nucleus and cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 8125 Human
- Entrez Gene: 25379 Rat
- Omim: 600832 Human
- SwissProt: P39687 Human
- SwissProt: P49911 Rat
- Unigene: 458747 Human
- Unigene: 10123 Rat
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Alternative names
- acidic (leucine-rich) nuclear phosphoprotein 32 family, member A antibody
- Acidic leucine-rich nuclear phosphoprotein 32 family member A antibody
- Acidic nuclear phosphoprotein 32 family member A antibody
see all
Images
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Western blot - Anti-PHAP1 antibody (ab5992)All lanes : Anti-PHAP1 antibody (ab5992) at 2 µg/ml
Lane 1 : Daudi cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : HepG2 cell lysates
Lane 4 : K562 cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution -
Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)Immunofluorescent analysis of 4% paraformaldehydefixed Raji Cells labeling PHAP I with ab5992 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Western blot - Anti-PHAP1 antibody (ab5992)Western blot analysis of PHAP I expression in human Raji cell lysate with anti-PHAP I at 2µ g/ml (lane A) and 4µ g/ml (lane B), respectively. Western blot analysis of PHAP I expression in human Raji cell lysate with anti-PHAP I at 2 µg/ml (lane A) and 4 µg/ml (lane B), respectively. -
Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)Immunocytochemical analysis of Raji cells labeling PHAP1 with ab5992 at 2 μg/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab5992 has been referenced in 1 publication.
- de Souza CF et al. Creatine transporter immunolocalization in aged human and detached retinas. Invest Ophthalmol Vis Sci 53:1936-45 (2012). PubMed: 22410560