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    phap1-antibody-ab5992.pdf

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Cell Biology Apoptosis Intracellular Caspases etc Caspases
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Anti-PHAP1 antibody (ab5992)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

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Western blot - Anti-PHAP1 antibody (ab5992)
  • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)
  • Western blot - Anti-PHAP1 antibody (ab5992)
  • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)

Key features and details

  • Rabbit polyclonal to PHAP1
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human PHAP1 protein (ab101155)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-PHAP1 antibody
    See all PHAP1 primary antibodies
  • Description

    Rabbit polyclonal to PHAP1
  • Host species

    Rabbit
  • Specificity

    This antibody does not cross-react with PHAP12a or PHAPIII.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human PHAP1.
    (Peptide available as ab6241)

  • Positive control

    • WB: Daudi, HeLa, HepG2, K562 and Raji cell lysates. ICC/IF: Raji cells
  • General notes

    Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-mediated cell transformation.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-mediated cell transformation.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human PHAP1 protein (ab101155)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Rat
Application Abreviews Notes
ICC/IF
Use a concentration of 2 µg/ml.
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa.
Notes
ICC/IF
Use a concentration of 2 µg/ml.
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa.

Target

  • Function

    Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.
  • Tissue specificity

    Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala.
  • Sequence similarities

    Belongs to the ANP32 family.
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
  • Post-translational
    modifications

    Phosphorylated on serine residues.
    The N-terminus is blocked.
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  • Cellular localization

    Nucleus. Cytoplasm. Endoplasmic reticulum. Translocates to the cytoplasm during the process of neuritogenesis (By similarity). Shuttles between nucleus and cytoplasm.
  • Target information above from: UniProt accession P39687 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8125 Human
    • Entrez Gene: 25379 Rat
    • Omim: 600832 Human
    • SwissProt: P39687 Human
    • SwissProt: P49911 Rat
    • Unigene: 458747 Human
    • Unigene: 10123 Rat
    • Alternative names

      • acidic (leucine-rich) nuclear phosphoprotein 32 family, member A antibody
      • Acidic leucine-rich nuclear phosphoprotein 32 family member A antibody
      • Acidic nuclear phosphoprotein 32 family member A antibody
      • Acidic nuclear phosphoprotein pp32 antibody
      • AN32A_HUMAN antibody
      • ANP32A antibody
      • C15orf1 antibody
      • Cerebellar leucine rich acidic nuclear protein antibody
      • Hepatopoietin Cn antibody
      • HPPCn antibody
      • I1PP2A antibody
      • Inhibitor 1 of protein phosphatase 2A antibody
      • inhibitor-1 of protein phosphatase-2A antibody
      • Lanp antibody
      • Leucine rich acidic nuclear protein antibody
      • Leucine-rich acidic nuclear protein antibody
      • MAPM antibody
      • Mapmodulin antibody
      • MGC119787 antibody
      • MGC150373 antibody
      • PHAPI antibody
      • Potent heat stable protein phosphatase 2A inhibitor I1PP2A antibody
      • Potent heat-stable protein phosphatase 2A inhibitor I1PP2A antibody
      • PP32 antibody
      • Putative HLA DR associated protein I antibody
      • Putative HLA-DR-associated protein I antibody
      • Putative human HLA class II associated protein I antibody
      • Putative human HLA class II-associated protein antibody
      see all

    Images

    • Western blot - Anti-PHAP1 antibody (ab5992)
      Western blot - Anti-PHAP1 antibody (ab5992)
      All lanes : Anti-PHAP1 antibody (ab5992) at 2 µg/ml

      Lane 1 : Daudi cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : HepG2 cell lysates
      Lane 4 : K562 cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
    • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)
      Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)

      Immunofluorescent analysis of 4% paraformaldehydefixed Raji Cells labeling PHAP I with ab5992 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

    • Western blot - Anti-PHAP1 antibody (ab5992)
      Western blot - Anti-PHAP1 antibody (ab5992)
      Western blot analysis of PHAP I expression in human Raji cell lysate with anti-PHAP I at 2 µg/ml (lane A) and 4 µg/ml (lane B), respectively. Western blot analysis of PHAP I expression in human Raji cell lysate with anti-PHAP I at 2 µg/ml (lane A) and 4 µg/ml (lane B), respectively.
    • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)
      Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)

      Immunocytochemical analysis of Raji cells labeling PHAP1 with ab5992 at 2 μg/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab5992? Please let us know so that we can cite the reference in this datasheet.

    ab5992 has been referenced in 1 publication.

    • de Souza CF  et al. Creatine transporter immunolocalization in aged human and detached retinas. Invest Ophthalmol Vis Sci 53:1936-45 (2012). PubMed: 22410560

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I wish to buy the anti PHAPI antibody however I cant tell what the difference is between ab5992 and ab5991. Is there any difference?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 24 2004

    Answer

    Thank you for your patience in awaiting a response from us. Ab5992 and Ab5991 are both against the same protein, PHAP1, but the sequences were derived from two different locations; the intermediate domain (IN) and the C-terminus (CT) of the protein respectively.

    Read More

    Abcam Scientific Support

    Answered on Jun 30 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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