Anti-PHAP1 antibody (ab5992)

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Overview

  • Product name

    Anti-PHAP1 antibody
    See all PHAP1 primary antibodies

  • Description

    Rabbit polyclonal to PHAP1

  • Host species

    Rabbit

  • Specificity

    This antibody does not cross-react with PHAP12a or PHAPIII.

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide corresponding to Human PHAP1.
    (Peptide available as ab6241)

  • Positive control

    • WB: Daudi, HeLa, HepG2, K562 and Raji cell lysates. ICC/IF: Raji cells

  • General notes

    Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-mediated cell transformation.

Properties

Applications

Our Abpromise guarantee covers the use of ab5992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa.

Target

  • Function

    Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.

  • Tissue specificity

    Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala.

  • Sequence similarities

    Belongs to the ANP32 family.
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.

  • Post-translational
    modifications

    Phosphorylated on serine residues.
    The N-terminus is blocked.
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.

  • Cellular localization

    Nucleus. Cytoplasm. Endoplasmic reticulum. Translocates to the cytoplasm during the process of neuritogenesis (By similarity). Shuttles between nucleus and cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • acidic (leucine-rich) nuclear phosphoprotein 32 family, member A antibody
    • Acidic leucine-rich nuclear phosphoprotein 32 family member A antibody
    • Acidic nuclear phosphoprotein 32 family member A antibody
    • Acidic nuclear phosphoprotein pp32 antibody
    • AN32A_HUMAN antibody
    • ANP32A antibody
    • C15orf1 antibody
    • Cerebellar leucine rich acidic nuclear protein antibody
    • Hepatopoietin Cn antibody
    • HPPCn antibody
    • I1PP2A antibody
    • Inhibitor 1 of protein phosphatase 2A antibody
    • inhibitor-1 of protein phosphatase-2A antibody
    • Lanp antibody
    • Leucine rich acidic nuclear protein antibody
    • Leucine-rich acidic nuclear protein antibody
    • MAPM antibody
    • Mapmodulin antibody
    • MGC119787 antibody
    • MGC150373 antibody
    • PHAPI antibody
    • Potent heat stable protein phosphatase 2A inhibitor I1PP2A antibody
    • Potent heat-stable protein phosphatase 2A inhibitor I1PP2A antibody
    • PP32 antibody
    • Putative HLA DR associated protein I antibody
    • Putative HLA-DR-associated protein I antibody
    • Putative human HLA class II associated protein I antibody
    • Putative human HLA class II-associated protein antibody
    see all

Images

  • Western blot - Anti-PHAP1 antibody (ab5992)
    Western blot - Anti-PHAP1 antibody (ab5992)
    All lanes : Anti-PHAP1 antibody (ab5992) at 2 µg/ml

    Lane 1 : Daudi cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : HepG2 cell lysates
    Lane 4 : K562 cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
  • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)
    Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)

    Immunofluorescent analysis of 4% paraformaldehydefixed Raji Cells labeling PHAP I with ab5992 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

  • Western blot - Anti-PHAP1 antibody (ab5992)
    Western blot - Anti-PHAP1 antibody (ab5992)
    Western blot analysis of PHAP I expression in human Raji cell lysate with anti-PHAP I at 2 µg/ml (lane A) and 4 µg/ml (lane B), respectively. Western blot analysis of PHAP I expression in human Raji cell lysate with anti-PHAP I at 2 µg/ml (lane A) and 4 µg/ml (lane B), respectively.
  • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)
    Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)

    Immunocytochemical analysis of Raji cells labeling PHAP1 with ab5992 at 2 μg/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

References

This product has been referenced in:

  • de Souza CF  et al. Creatine transporter immunolocalization in aged human and detached retinas. Invest Ophthalmol Vis Sci 53:1936-45 (2012). Read more (PubMed: 22410560) »
See 1 Publication for this product

Publishing research using ab5992? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

I wish to buy the anti PHAPI antibody however I cant tell what the difference is between ab5992 and ab5991. Is there any difference?

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Answer

Thank you for your patience in awaiting a response from us. Ab5992 and Ab5991 are both against the same protein, PHAP1, but the sequences were derived from two different locations; the intermediate domain (IN) and the C-terminus (CT) of the protein respectively.

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