Overview

  • Product name

    Anti-PHAPI2 / APRIL antibody [EPR14589]
    See all PHAPI2 / APRIL primary antibodies
  • Description

    Rabbit monoclonal [EPR14589] to PHAPI2 / APRIL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PHAPI2/ APRIL aa 100-200. The exact sequence is proprietary.
    Database link: Q92688

  • Positive control

    • LnCaP, PC3 and Jurkat cell lysates; PC3 cells; Human pancreas and Human prostatic hyperplasia tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome.
  • Tissue specificity

    Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta.
  • Sequence similarities

    Belongs to the ANP32 family.
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
  • Domain

    Histone binding is mediated by the concave surface of the LRR region.
  • Post-translational
    modifications

    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  • Cellular localization

    Nucleus. Accumulates in the nuclei at the S phase and Cytoplasm. Lacks a nuclear localization signal.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acidic (leucine rich) nuclear phosphoprotein 32 family member B antibody
    • Acidic leucine rich nuclear phosphoprotein 32 family member B antibody
    • Acidic leucine-rich nuclear phosphoprotein 32 family member B antibody
    • Acidic nuclear phosphoprotein 32 family member B antibody
    • Acidic protein rich in leucines antibody
    • AN32B_HUMAN antibody
    • ANP 32B antibody
    • ANP32B antibody
    • APRIL antibody
    • OTTHUMP0000006377 antibody
    • PAL 31 antibody
    • PAL31 antibody
    • PHAPI 2 antibody
    • PHAPI2 antibody
    • PHAPI2 protein antibody
    • PHAPI2a antibody
    • Proliferation related acidic leucine rich protein antibody
    • Putative HLA-DR-associated protein I-2 antibody
    • Silver stainable protein SSP 29 antibody
    • Silver stainable protein SSP29 antibody
    • Silver-stainable protein SSP29 antibody
    • Ssp 29 antibody
    • Ssp29 antibody
    see all

Images

  • All lanes : Anti-PHAPI2 / APRIL antibody [EPR14589] (ab184565) at 1/5000 dilution

    Lane 1 : LnCaP cell lysate
    Lane 2 : PC3 cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 29 kDa

  • Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling PHAPI2 / APRIL with ab184565 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow cytometric analysis of PC3 cells (2% paraformaldehyde-fixed) labeling PHAPI2 / APRIL with ab184565 at 1/20 dilution (red) or Rabbit monoclonal IgG isotype control (green) followed by Goat anti rabbit IgG (FITC) at 1/150 dilution.

  • Immunofluorescent analysis of PC3 cells (4% pafarormaldehyde-fixed) labeling PHAPI2 / APRIL with ab184565 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution and DAPI staining (blue).

  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PHAPI2 / APRIL with ab184565 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Domingues P  et al. Profiling host ANP32A splicing landscapes to predict influenza A virus polymerase adaptation. Nat Commun 10:3396 (2019). Read more (PubMed: 31363119) »
  • Long JS  et al. Species difference in ANP32A underlies influenza A virus polymerase host restriction. Nature 529:101-4 (2016). WB . Read more (PubMed: 26738596) »
See all 2 Publications for this product

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