Overview

  • Product name
    Anti-PHAX antibody - C-terminal
    See all PHAX primary antibodies
  • Description
    Rabbit polyclonal to PHAX - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PHAX aa 304-334 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9H814

  • Positive control
    • NCI-H460 cell line lysates; Human brain tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab171321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 44 kDa.

Target

  • Function
    A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner (By similarity). Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA.
  • Sequence similarities
    Belongs to the PHAX family.
  • Post-translational
    modifications
    Phosphorylated in the nucleus. Dephosphorylated in the cytoplasm (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus > nucleoplasm. Nucleus > Cajal body. Cytoplasm. Located in the nucleoplasm and Cajal bodies. Shuttles between the nucleus and the cytoplasm. Shuttles between the nucleoplasm and Cajal bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ13193 antibody
    • PHAX antibody
    • PHAX_HUMAN antibody
    • Phosphorylated adapter RNA export protein antibody
    • Phosphorylated adaptor for RNA export antibody
    • RNA U small nuclear RNA export adapter protein antibody
    • RNA U, small nuclear RNA export adapter (phosphorylation regulated) antibody
    • RNUXA antibody
    see all

Images

  • Anti-PHAX antibody - C-terminal (ab171321) at 1/100 dilution + NCI-H460 cell line lysates at 35 µg

    Predicted band size: 44 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling PHAX with ab171321 at 1/10 dilution. Detection utilised peroxidase conjugation of the secondary antibody and DAB staining.

References

ab171321 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab171321.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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