Recombinant Anti-PH3 antibody [EPR13306] (ab180577)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13306] to PH3
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-PH3 antibody [EPR13306]
See all PH3 primary antibodies -
Description
Rabbit monoclonal [EPR13306] to PH3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HepG2, SH-SY5Y, HeLa and 293 cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13306 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
|
Notes |
---|
WB
1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa). |
Target
-
Function
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. -
Sequence similarities
Contains 1 FCS-type zinc finger.
Contains 1 SAM (sterile alpha motif) domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 80012 Human
- Entrez Gene: 241915 Mouse
- Entrez Gene: 310258 Rat
- SwissProt: Q8NDX5 Human
- SwissProt: Q8CHP6 Mouse
- Unigene: 529592 Human
- Unigene: 694467 Human
- Unigene: 233173 Mouse
-
Alternative names
- Early development regulator 3 antibody
- Early development regulatory protein 3 antibody
- EDR3 antibody
see all
Images
-
All lanes : Anti-PH3 antibody [EPR13306] (ab180577) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 106 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab180577 has not yet been referenced specifically in any publications.