1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May play a role in transcriptional regulation.
Involvement in disease
Defects in PHF6 are the cause of Boerjeson-Forssman-Lehmann syndrome (BFLS) [MIM:301900]; also known as Boerjeson-Forssman syndrome (BORJ). BFLS is a X-linked recessive disorder characterized by moderate to severe mental retardation, epilepsy, hypogonadism, hypometabolism, obesity with marked gynecomastia, swelling of subcutaneous tissue of the face, narrow palpebral fissure and large but not deformed ears.
Contains 2 PHD-type zinc fingers.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus. Nucleus > nucleolus. Nuclear, it particularly localizes to the nucleolus.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PHD finger protein 6 with ab99398 at 1/200 (1µg/ml). Detection: DAB.
Western blot - Anti-PHD finger protein 6 antibody (ab99398)
All lanes : Anti-PHD finger protein 6 antibody (ab99398) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysates at 50 µg Lane 2 : HeLa whole cell lysates at 15 µg Lane 3 : HeLa whole cell lysates at 5 µg Lane 4 : 293T whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 10 seconds
Immunoprecipitation - Anti-PHD finger protein 6 antibody (ab99398)
Detection of PHD finger protein 6 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99398 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with exposure time of 3 seconds.