Recombinant
RabMAb

Anti-PHD finger protein 6 antibody [EPR11997] (ab173304)

Overview

  • Product name
    Anti-PHD finger protein 6 antibody [EPR11997]
    See all PHD finger protein 6 primary antibodies
  • Description
    Rabbit monoclonal [EPR11997] to PHD finger protein 6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PHD finger protein 6 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8IWS0

  • Positive control
    • HeLa, K562, and 293T cell lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11997
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab173304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 41 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      May play a role in transcriptional regulation.
    • Tissue specificity
      Ubiquitously expressed.
    • Involvement in disease
      Defects in PHF6 are the cause of Boerjeson-Forssman-Lehmann syndrome (BFLS) [MIM:301900]; also known as Boerjeson-Forssman syndrome (BORJ). BFLS is a X-linked recessive disorder characterized by moderate to severe mental retardation, epilepsy, hypogonadism, hypometabolism, obesity with marked gynecomastia, swelling of subcutaneous tissue of the face, narrow palpebral fissure and large but not deformed ears.
    • Sequence similarities
      Contains 2 PHD-type zinc fingers.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus. Nucleus > nucleolus. Nuclear, it particularly localizes to the nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • AC004383.6 antibody
      • BFLS antibody
      • BORJ antibody
      • CENP 31 antibody
      • Centromere protein 31 antibody
      • MGC14797 antibody
      • OTTHUMP00000024063 antibody
      • PHD finger protein 6 antibody
      • PHD like zinc finger protein antibody
      • PHD-like zinc finger protein antibody
      • Phf6 antibody
      • PHF6_HUMAN antibody
      see all

    Images

    • Immunofluorescent analysis of HeLa cells labeling PHD finger protein 6 with ab173304 at 1/50 dilution.

    • All lanes : Anti-PHD finger protein 6 antibody [EPR11997] (ab173304) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 41 kDa

    • Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab173304.

    References

    ab173304 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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