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  1. Link

    phd-finger-protein-6phf6-antibody-ab111844.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-PHD finger protein 6/PHF6 antibody (ab111844)

  • Datasheet
  • SDS
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  • Western blot - Anti-PHD finger protein 6/PHF6 antibody (ab111844)
  • Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 6/PHF6 antibody (ab111844)

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-PHD finger protein 6/PHF6 antibody
    See all PHD finger protein 6/PHF6 primary antibodies
  • Description

    Rabbit polyclonal to PHD finger protein 6/PHF6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Pig
  • Immunogen

    Recombinant fragment corresponding to Human PHD finger protein 6/PHF6 aa 66-251. (AAH05994)

  • Positive control

    • NT2D1, IMR32, U-87 MG, MCF7 cells and whole cell lysates.
  • General notes

     This product was previously labelled as PHD finger protein 6

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab111844 is purified by antigen-affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • MCF7 nuclear extract lysate (ab14860)
    • U87 MG nuclear extract lysate (ab14903)
    • MCF7 whole cell lysate (ab29537)
    • MCF7 whole cell lysate (ab3871)
  • Recombinant Protein

    • Recombinant Human PHD finger protein 6/PHF6 (ab164560)

Applications

Our Abpromise guarantee covers the use of ab111844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.
ICC/IF 1/100 - 1/500.

Target

  • Function

    May play a role in transcriptional regulation.
  • Tissue specificity

    Ubiquitously expressed.
  • Involvement in disease

    Defects in PHF6 are the cause of Boerjeson-Forssman-Lehmann syndrome (BFLS) [MIM:301900]; also known as Boerjeson-Forssman syndrome (BORJ). BFLS is a X-linked recessive disorder characterized by moderate to severe mental retardation, epilepsy, hypogonadism, hypometabolism, obesity with marked gynecomastia, swelling of subcutaneous tissue of the face, narrow palpebral fissure and large but not deformed ears.
  • Sequence similarities

    Contains 2 PHD-type zinc fingers.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Nucleus > nucleolus. Nuclear, it particularly localizes to the nucleolus.
  • Target information above from: UniProt accession Q8IWS0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 84295 Human
    • Entrez Gene: 70998 Mouse
    • Omim: 300414 Human
    • SwissProt: Q8IWS0 Human
    • SwissProt: Q9D4J7 Mouse
    • Unigene: 356501 Human
    • Unigene: 26870 Mouse
    • Alternative names

      • AC004383.6 antibody
      • BFLS antibody
      • BORJ antibody
      • CENP 31 antibody
      • Centromere protein 31 antibody
      • MGC14797 antibody
      • OTTHUMP00000024063 antibody
      • PHD finger protein 6 antibody
      • PHD like zinc finger protein antibody
      • PHD-like zinc finger protein antibody
      • Phf6 antibody
      • PHF6_HUMAN antibody
      see all

    Images

    • Western blot - Anti-PHD finger protein 6/PHF6 antibody (ab111844)
      Western blot - Anti-PHD finger protein 6/PHF6 antibody (ab111844)
      Anti-PHD finger protein 6/PHF6 antibody (ab111844) at 1/1000 dilution + MCF 7 whole cell lysate at 30 µg

      Predicted band size: 35 kDa



      10% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 6/PHF6 antibody (ab111844)
      Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 6/PHF6 antibody (ab111844)

      ab111844 at 1/500 dilution staining PHD finger protein 6/PHF6 in Paraformaldehyde-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References

    ab111844 has not yet been referenced specifically in any publications.

    Publishing research using ab111844? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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