Anti-PHD finger protein 6/PHF6 antibody (ab111844)
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Overview
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Product name
Anti-PHD finger protein 6/PHF6 antibody
See all PHD finger protein 6/PHF6 primary antibodies -
Description
Rabbit polyclonal to PHD finger protein 6/PHF6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Pig
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Immunogen
Recombinant fragment corresponding to Human PHD finger protein 6/PHF6 aa 66-251. (AAH05994)
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Positive control
- NT2D1, IMR32, U-87 MG, MCF7 cells and whole cell lysates.
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General notes
This product was previously labelled as PHD finger protein 6
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab111844 is purified by antigen-affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab111844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 35 kDa. | |
| ICC/IF | 1/100 - 1/500. |
Target
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Function
May play a role in transcriptional regulation. -
Tissue specificity
Ubiquitously expressed. -
Involvement in disease
Defects in PHF6 are the cause of Boerjeson-Forssman-Lehmann syndrome (BFLS) [MIM:301900]; also known as Boerjeson-Forssman syndrome (BORJ). BFLS is a X-linked recessive disorder characterized by moderate to severe mental retardation, epilepsy, hypogonadism, hypometabolism, obesity with marked gynecomastia, swelling of subcutaneous tissue of the face, narrow palpebral fissure and large but not deformed ears. -
Sequence similarities
Contains 2 PHD-type zinc fingers. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Nucleus > nucleolus. Nuclear, it particularly localizes to the nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 84295 Human
- Entrez Gene: 70998 Mouse
- Omim: 300414 Human
- SwissProt: Q8IWS0 Human
- SwissProt: Q9D4J7 Mouse
- Unigene: 356501 Human
- Unigene: 26870 Mouse
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Alternative names
- AC004383.6 antibody
- BFLS antibody
- BORJ antibody
see all
Images
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Western blot - Anti-PHD finger protein 6/PHF6 antibody (ab111844)Anti-PHD finger protein 6/PHF6 antibody (ab111844) at 1/1000 dilution + MCF 7 whole cell lysate at 30 µg
Predicted band size: 35 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 6/PHF6 antibody (ab111844)ab111844 at 1/500 dilution staining PHD finger protein 6/PHF6 in Paraformaldehyde-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
References
ab111844 has not yet been referenced specifically in any publications.
