Anti-PHD finger protein 6/PHF6 antibody (ab111844)
Key features and details
- Rabbit polyclonal to PHD finger protein 6/PHF6
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PHD finger protein 6/PHF6 antibody
See all PHD finger protein 6/PHF6 primary antibodies -
Description
Rabbit polyclonal to PHD finger protein 6/PHF6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Pig
-
Immunogen
Recombinant fragment corresponding to Human PHD finger protein 6/PHF6 aa 66-251. (AAH05994)
-
Positive control
- NT2D1, IMR32, U-87 MG, MCF7 cells and whole cell lysates.
-
General notes
This product was previously labelled as PHD finger protein 6
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab111844 is purified by antigen-affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab111844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 35 kDa. | |
| ICC/IF | 1/100 - 1/500. |
Target
-
Function
May play a role in transcriptional regulation. -
Tissue specificity
Ubiquitously expressed. -
Involvement in disease
Defects in PHF6 are the cause of Boerjeson-Forssman-Lehmann syndrome (BFLS) [MIM:301900]; also known as Boerjeson-Forssman syndrome (BORJ). BFLS is a X-linked recessive disorder characterized by moderate to severe mental retardation, epilepsy, hypogonadism, hypometabolism, obesity with marked gynecomastia, swelling of subcutaneous tissue of the face, narrow palpebral fissure and large but not deformed ears. -
Sequence similarities
Contains 2 PHD-type zinc fingers. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Nucleus > nucleolus. Nuclear, it particularly localizes to the nucleolus. - Information by UniProt
-
Database links
- Entrez Gene: 84295 Human
- Entrez Gene: 70998 Mouse
- Omim: 300414 Human
- SwissProt: Q8IWS0 Human
- SwissProt: Q9D4J7 Mouse
- Unigene: 356501 Human
- Unigene: 26870 Mouse
-
Alternative names
- AC004383.6 antibody
- BFLS antibody
- BORJ antibody
see all
Images
-
Western blot - Anti-PHD finger protein 6/PHF6 antibody (ab111844)Anti-PHD finger protein 6/PHF6 antibody (ab111844) at 1/1000 dilution + MCF 7 whole cell lysate at 30 µg
Predicted band size: 35 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 6/PHF6 antibody (ab111844)ab111844 at 1/500 dilution staining PHD finger protein 6/PHF6 in Paraformaldehyde-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
Protocols
References (0)
ab111844 has not yet been referenced specifically in any publications.
