Key features and details
- Rabbit polyclonal to PHD finger protein 6/PHF6
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PHD finger protein 6/PHF6 antibody
See all PHD finger protein 6/PHF6 primary antibodies
DescriptionRabbit polyclonal to PHD finger protein 6/PHF6
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Pig
Recombinant fragment corresponding to Human PHD finger protein 6/PHF6 aa 66-251. (AAH05994)
- NT2D1, IMR32, U-87 MG, MCF7 cells and whole cell lysates.
This product was previously labelled as PHD finger protein 6
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab111844 is purified by antigen-affinity chromatography.
Our Abpromise guarantee covers the use of ab111844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 35 kDa.|
|ICC/IF||1/100 - 1/500.|
FunctionMay play a role in transcriptional regulation.
Tissue specificityUbiquitously expressed.
Involvement in diseaseDefects in PHF6 are the cause of Boerjeson-Forssman-Lehmann syndrome (BFLS) [MIM:301900]; also known as Boerjeson-Forssman syndrome (BORJ). BFLS is a X-linked recessive disorder characterized by moderate to severe mental retardation, epilepsy, hypogonadism, hypometabolism, obesity with marked gynecomastia, swelling of subcutaneous tissue of the face, narrow palpebral fissure and large but not deformed ears.
Sequence similaritiesContains 2 PHD-type zinc fingers.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Nucleus > nucleolus. Nuclear, it particularly localizes to the nucleolus.
- Information by UniProt
- AC004383.6 antibody
- BFLS antibody
- BORJ antibody
Anti-PHD finger protein 6/PHF6 antibody (ab111844) at 1/1000 dilution + MCF 7 whole cell lysate at 30 µg
Predicted band size: 35 kDa
10% SDS PAGE
ab111844 at 1/500 dilution staining PHD finger protein 6/PHF6 in Paraformaldehyde-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
ab111844 has not yet been referenced specifically in any publications.