Product nameAnti-PHD finger protein 6/PHF6 antibody
See all PHD finger protein 6/PHF6 primary antibodies
DescriptionRabbit polyclonal to PHD finger protein 6/PHF6
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Orangutan
Synthetic peptide within Human PHD finger protein 6/PHF6 aa 125-175. The exact sequence is proprietary.
Database link: Q8IWS0
- IHC-P: Human breast carcinoma tissue. WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264208 in the following tested applications.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000.|
FunctionMay play a role in transcriptional regulation.
Tissue specificityUbiquitously expressed.
Involvement in diseaseDefects in PHF6 are the cause of Boerjeson-Forssman-Lehmann syndrome (BFLS) [MIM:301900]; also known as Boerjeson-Forssman syndrome (BORJ). BFLS is a X-linked recessive disorder characterized by moderate to severe mental retardation, epilepsy, hypogonadism, hypometabolism, obesity with marked gynecomastia, swelling of subcutaneous tissue of the face, narrow palpebral fissure and large but not deformed ears.
Sequence similaritiesContains 2 PHD-type zinc fingers.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Nucleus > nucleolus. Nuclear, it particularly localizes to the nucleolus.
- Information by UniProt
- AC004383.6 antibody
- BFLS antibody
- BORJ antibody
All lanes : Anti-PHD finger protein 6/PHF6 antibody (ab264208) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 3 seconds
Formalin-fixed, paraffin-embedded human breast cancer carcinoma tissue stained for PHD finger protein 6/PHF6 using ab264208 at 1/200 dilution in immunohistochemical analysis. Detection: DAB.
PHD finger protein 6/PHF6 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264208 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264208 at 1 µg/ml.
Lane 1: ab264208 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
ab264208 has not yet been referenced specifically in any publications.