Recombinant Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11997] to PHD finger protein 6/PHF6
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-PHD finger protein 6/PHF6 antibody [EPR11997]
See all PHD finger protein 6/PHF6 primary antibodies -
Description
Rabbit monoclonal [EPR11997] to PHD finger protein 6/PHF6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HeLa, A431, HEK-293, K562, and 293T cell lysates; HeLa cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11997 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab173304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 41 kDa.
|
|
ICC/IF |
1/50 - 1/100.
|
Notes |
---|
WB
1/1000 - 1/5000. Predicted molecular weight: 41 kDa. |
ICC/IF
1/50 - 1/100. |
Target
-
Function
May play a role in transcriptional regulation. -
Tissue specificity
Ubiquitously expressed. -
Involvement in disease
Defects in PHF6 are the cause of Boerjeson-Forssman-Lehmann syndrome (BFLS) [MIM:301900]; also known as Boerjeson-Forssman syndrome (BORJ). BFLS is a X-linked recessive disorder characterized by moderate to severe mental retardation, epilepsy, hypogonadism, hypometabolism, obesity with marked gynecomastia, swelling of subcutaneous tissue of the face, narrow palpebral fissure and large but not deformed ears. -
Sequence similarities
Contains 2 PHD-type zinc fingers. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Nucleus > nucleolus. Nuclear, it particularly localizes to the nucleolus. - Information by UniProt
-
Database links
- Entrez Gene: 84295 Human
- Entrez Gene: 70998 Mouse
- Entrez Gene: 100359714 Rat
- Omim: 300414 Human
- SwissProt: Q8IWS0 Human
- SwissProt: Q9D4J7 Mouse
- Unigene: 356501 Human
- Unigene: 26870 Mouse
-
Alternative names
- AC004383.6 antibody
- BFLS antibody
- BORJ antibody
see all
Images
-
All lanes : Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304) at 1/1000 dilution
Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : PHF6 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under non-reducing conditions.
Predicted band size: 41 kDaLanes 1 - 4: Merged signal (red and green). Green - ab173304 observed at 41 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab173304 was shown to recognize PHD finger protein 6 in wild-type HEK-293 cells as signal was lost at the expected MW in PHF6 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PHF6 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab173304 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
All lanes : Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 41 kDa -
Immunofluorescent analysis of HeLa cells labeling PHD finger protein 6/PHF6 with ab173304 at 1/50 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab173304 has been referenced in 1 publication.
- Cai T et al. Notoginsenoside R1 induces DNA damage via PHF6 protein to inhibit cervical carcinoma cell proliferation. Mol Med Rep 23:N/A (2021). PubMed: 33537816