Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)

Overview

  • Product name

    Anti-PHD finger protein 6/PHF6 antibody [EPR11997]
    See all PHD finger protein 6/PHF6 primary antibodies
  • Description

    Rabbit monoclonal [EPR11997] to PHD finger protein 6/PHF6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human PHD finger protein 6/PHF6 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8IWS0

  • Positive control

    • HeLa, A431, HEK-293, K562, and 293T cell lysates; HeLa cells.
  • General notes

     

    This product was previously labelled as PHD finger protein 6

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11997
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab173304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 41 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      May play a role in transcriptional regulation.
    • Tissue specificity

      Ubiquitously expressed.
    • Involvement in disease

      Defects in PHF6 are the cause of Boerjeson-Forssman-Lehmann syndrome (BFLS) [MIM:301900]; also known as Boerjeson-Forssman syndrome (BORJ). BFLS is a X-linked recessive disorder characterized by moderate to severe mental retardation, epilepsy, hypogonadism, hypometabolism, obesity with marked gynecomastia, swelling of subcutaneous tissue of the face, narrow palpebral fissure and large but not deformed ears.
    • Sequence similarities

      Contains 2 PHD-type zinc fingers.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus. Nucleus > nucleolus. Nuclear, it particularly localizes to the nucleolus.
    • Information by UniProt
    • Database links

    • Alternative names

      • AC004383.6 antibody
      • BFLS antibody
      • BORJ antibody
      • CENP 31 antibody
      • Centromere protein 31 antibody
      • MGC14797 antibody
      • OTTHUMP00000024063 antibody
      • PHD finger protein 6 antibody
      • PHD like zinc finger protein antibody
      • PHD-like zinc finger protein antibody
      • Phf6 antibody
      • PHF6_HUMAN antibody
      see all

    Images

    • All lanes : Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304) at 1/1000 dilution

      Lane 1 : Wild-type HEK-293 whole cell lysate
      Lane 2 : PHF6 knockout HEK-293 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : A431 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 41 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab173304 observed at 41 kDa. Red - loading control, ab130007, observed at 130 kDa.

      ab173304 was shown to recognize PHD finger protein 6 in wild-type HEK-293 cells as signal was lost at the expected MW in PHF6 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PHF6 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab173304 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 41 kDa

    • Immunofluorescent analysis of HeLa cells labeling PHD finger protein 6/PHF6 with ab173304 at 1/50 dilution.

    • Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab173304.

    References

    ab173304 has not yet been referenced specifically in any publications.

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