Overview

  • Product name

    Anti-PHD1/prolyl hydroxylase antibody
    See all PHD1/prolyl hydroxylase primary antibodies
  • Description

    Rabbit polyclonal to PHD1/prolyl hydroxylase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 141-387 of Human PHD1/prolyl hydroxylase (Q96KS0).

  • Positive control

    • Human lung adenocarcinoma tissue; HeLa cells; A549, HeLa, HepG2 and HCT116 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab127905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation. Isoform p40 and isoform p43 exhibit the same level of activity.
  • Tissue specificity

    Expressed abundantly in all tissues with highest expression in testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium.
  • Sequence similarities

    Contains 1 Fe2OG dioxygenase domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp434E026 antibody
    • EGL nine (C.elegans) homolog 2 antibody
    • Egl nine homolog 2 (C. elegans) antibody
    • Egl nine homolog 2 antibody
    • EGLN 2 antibody
    • EGLN2 antibody
    • EGLN2_HUMAN antibody
    • EIT 6 antibody
    • EIT6 antibody
    • Estrogen-induced tag 6 antibody
    • HIF P4H 1 antibody
    • HIF PH1 antibody
    • HIF prolyl hydroxylase 1 antibody
    • HIF-PH1 antibody
    • HIF-prolyl hydroxylase 1 antibody
    • HIFPH 1 antibody
    • HIFPH1 antibody
    • HPH 3 antibody
    • HPH-1 antibody
    • HPH-3 antibody
    • HPH3 antibody
    • Hypoxia inducible factor prolyl hydroxylase 1 antibody
    • Hypoxia-inducible factor prolyl hydroxylase 1 antibody
    • P4H1 antibody
    • PHD 1 antibody
    • PhD1 antibody
    • prolyl hydroxylase domain containing protein 1 antibody
    • Prolyl hydroxylase domain-containing protein 1 antibody
    see all

Images

  • Anti-PHD1/prolyl hydroxylase antibody (ab127905) at 1/5000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 44 kDa



    10% SDS Page
  • ab127905, at a 1/500 dilution, staining PHD1/prolyl hydroxylase in paraformaldehyde-fixed HeLa cells by Immunofluorescence (green). Alpha-tubulin filaments are stained in red and the nucleus in blue.
  • ab127905, at a 1/250 dilution, staining PHD1/prolyl hydroxylase in paraffin-embedded Human lung adenocarcinoma tissue by immunohistochemistry.

References

ab127905 has not yet been referenced specifically in any publications.

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