Key features and details
- Rabbit polyclonal to PHD1/prolyl hydroxylase
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PHD1/prolyl hydroxylase antibody
See all PHD1/prolyl hydroxylase primary antibodies
DescriptionRabbit polyclonal to PHD1/prolyl hydroxylase
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 141-387 of Human PHD1/prolyl hydroxylase (Q96KS0).
- Human lung adenocarcinoma tissue; HeLa cells; A549, HeLa, HepG2 and HCT116 whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab127905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 44 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).|
|ICC/IF||1/100 - 1/1000.|
FunctionCatalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation. Isoform p40 and isoform p43 exhibit the same level of activity.
Tissue specificityExpressed abundantly in all tissues with highest expression in testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium.
Sequence similaritiesContains 1 Fe2OG dioxygenase domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- DKFZp434E026 antibody
- EGL nine (C.elegans) homolog 2 antibody
- Egl nine homolog 2 (C. elegans) antibody
Anti-PHD1/prolyl hydroxylase antibody (ab127905) at 1/5000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 44 kDa
10% SDS Page
ab127905, at a 1/500 dilution, staining PHD1/prolyl hydroxylase in paraformaldehyde-fixed HeLa cells by Immunofluorescence (green). Alpha-tubulin filaments are stained in red and the nucleus in blue.
ab127905, at a 1/250 dilution, staining PHD1/prolyl hydroxylase in paraffin-embedded Human lung adenocarcinoma tissue by immunohistochemistry.
ab127905 has not yet been referenced specifically in any publications.