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Your data sheet states this needs antigen retrieval- this is not correct as the originator of this mAB I have validated it for IHC and it will not work if you use antigen retrieval
Asked on Jun 12 2012
Thank you for your enquiry regarding ab82884.
This particular antibody has not been tested for IHC-P in the lab as standard procedure, however based on this specific publication it is suitable for this application. I would advise you to take a look at this published work for further information:
Andersen S et al. Overexpression of the HIF Hydroxylases PHD1, PHD2, PHD3 and FIH Are Individually and Collectively Unfavorable Prognosticators for NSCLC Survival. PLoS One 6:e23847 (2011). IHC-P; Human.
I hope this helps and if I can assist further, please do not hesitate to contact me.
Answered on Jun 12 2012