Overview

  • Product name

    Anti-PHD2 / prolyl hydroxylase antibody - C-terminal
    See all PHD2 / prolyl hydroxylase primary antibodies
  • Description

    Rabbit polyclonal to PHD2 / prolyl hydroxylase - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PHD2/ prolyl hydroxylase (C terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
    Database link: Q9GZT9

  • Positive control

    • IHC-Fr: Adult mouse cerebellum tissue. WB: HeLa, untreated and treated with cobalt (II) chloride, whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab226890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-Fr 1/100 - 1/1000.

Target

  • Function

    Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
  • Tissue specificity

    According to PubMed:11056053, widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to PubMed:12351678 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle.
  • Involvement in disease

    Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3) [MIM:609820]. ECYT3 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated serum hemoglobin and hematocrit, and normal serum erythropoietin levels.
  • Sequence similarities

    Contains 1 Fe2OG dioxygenase domain.
    Contains 1 MYND-type zinc finger.
  • Information by UniProt
  • Database links

  • Alternative names

    • C1ORF12 antibody
    • Chromosome 1 Open Reading Frame 12 antibody
    • DKFZp761F179 antibody
    • ECYT 3 antibody
    • ECYT3 antibody
    • Egl 9 family hypoxia inducible factor 1 antibody
    • EGL 9 homolog of C. elegans 1 antibody
    • EGL nine (C.elegans) homolog 1 antibody
    • Egl nine homolog 1 (C. elegans) antibody
    • Egl nine homolog 1 antibody
    • Egl nine like protein 1 antibody
    • EGLN 1 antibody
    • Egln1 antibody
    • EGLN1_HUMAN antibody
    • HIF PH2 antibody
    • HIF Prolyl Hydroxylase 2 antibody
    • HIF-PH2 antibody
    • HIF-prolyl hydroxylase 2 antibody
    • HIFP4H 2 antibody
    • HIFPH2 antibody
    • HPH 2 antibody
    • HPH-2 antibody
    • HPH2 antibody
    • Hypoxia inducible factor prolyl hydroxylase 2 antibody
    • Hypoxia-inducible factor prolyl hydroxylase 2 antibody
    • ORF13 antibody
    • P4H2 antibody
    • PHD 2 antibody
    • PhD2 antibody
    • PNAS 118 antibody
    • PNAS 137 antibody
    • Prolyl Hydroxylase Domain Containing Protein 2 antibody
    • Prolyl hydroxylase domain-containing protein 2 antibody
    • Rat Homolog of SM20 antibody
    • SM 20 antibody
    • SM-20 antibody
    • SM20 antibody
    • Zinc finger MYND domain containing protein 6 antibody
    • ZMYND6 antibody
    see all

Images

  • Frozen-sectioned adult mouse cerebellum tissue stained for PHD2 / prolyl hydroxylase using ab226890 at 1/250 dilution in immunohistochemical analysis.

    Red: NF-H, stained by NF-H antibody diluted at 1/500.
    Blue: DAPI.

  • All lanes : Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (ab226890) at 1/2000 dilution

    Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 200 µM cobalt (II) chloride for 24 hours whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 46 kDa



    10% SDS-PAGE gel.

References

ab226890 has not yet been referenced specifically in any publications.

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