Question (66815) | Anti-PHD3 antibody (ab30782)

Go to datasheet (ab30782)

Question

a customer of ours has bought the followong Abs:
ab30782
ab65979
ab110025
and she has to use them in IHC-P experiments.
She would like to know if for those Abs it is recommended an antigen retrieval.
If it is, can you please let me know the protocol (enzymatic or by heating)?
Incubation time and temperature ect....

Thanks in advance and kind regards

Answer

Thank you for contacting us.

As always, if we do not advise a certain method on our datasheet, the customer can use the one which they usually use within their lab. This is the case of ab110025.

For ab65979 I would suggest checking the publication which is cited within the application as we added IHC-P due to this application and haven't tested the antibody ourselves. Nonetheless, it is covered by our guarantee.

In the case of ab30782 we suggest a heat mediated antigen retrieval. The image description on the datasheet mentions that citrate buffer was used, but if the customer prefers to use EDTA it should be fine as well.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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