Anti-PHD4/prolyl hydroxylase antibody (ab126943)
Key features and details
- Rabbit polyclonal to PHD4/prolyl hydroxylase
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PHD4/prolyl hydroxylase antibody
See all PHD4/prolyl hydroxylase primary antibodies -
Description
Rabbit polyclonal to PHD4/prolyl hydroxylase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PHD4/prolyl hydroxylase aa 99-342 (internal sequence).
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Positive control
- Human colon carcinoma tissue; A549, H1299 and HCT116 whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab126943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 57 kDa. | |
| IHC-P | 1/100 - 1/500. |
Target
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Function
Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. -
Tissue specificity
Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts. -
Sequence similarities
Contains 2 EF-hand domains.
Contains 1 Fe2OG dioxygenase domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 54681 Human
- Omim: 614584 Human
- SwissProt: Q9NXG6 Human
- Unigene: 654944 Human
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Alternative names
- EGLN4 antibody
- HIF PH4 antibody
- HIF Prolyl Hydroxylase 4 antibody
see all
Images
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Western blot - Anti-PHD4/prolyl hydroxylase antibody (ab126943)All lanes : Anti-PHD4/prolyl hydroxylase antibody (ab126943) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HCT116 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa
7.5% SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD4/prolyl hydroxylase antibody (ab126943)ab126943, at 1/500 dilution, staining PHD4/prolyl hydroxylase in paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.
Protocols
References (0)
ab126943 has not yet been referenced specifically in any publications.
