Anti-PHD4/prolyl hydroxylase antibody (ab126943)
- Datasheet
- References
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Overview
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Product name
Anti-PHD4/prolyl hydroxylase antibody
See all PHD4/prolyl hydroxylase primary antibodies -
Description
Rabbit polyclonal to PHD4/prolyl hydroxylase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PHD4/prolyl hydroxylase aa 99-342 (internal sequence).
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Positive control
- Human colon carcinoma tissue; A549, H1299 and HCT116 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab126943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 57 kDa. | |
| IHC-P | 1/100 - 1/500. |
Target
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Function
Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. -
Tissue specificity
Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts. -
Sequence similarities
Contains 2 EF-hand domains.
Contains 1 Fe2OG dioxygenase domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 54681 Human
- Omim: 614584 Human
- SwissProt: Q9NXG6 Human
- Unigene: 654944 Human
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Alternative names
- EGLN4 antibody
- HIF PH4 antibody
- HIF Prolyl Hydroxylase 4 antibody
see all
Images
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Western blot - Anti-PHD4/prolyl hydroxylase antibody (ab126943)All lanes : Anti-PHD4/prolyl hydroxylase antibody (ab126943) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HCT116 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa
7.5% SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD4/prolyl hydroxylase antibody (ab126943)ab126943, at 1/500 dilution, staining PHD4/prolyl hydroxylase in paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.
References
ab126943 has not yet been referenced specifically in any publications.
