Key features and details
- Mouse polyclonal to PHD4/prolyl hydroxylase
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PHD4/prolyl hydroxylase antibody
See all PHD4/prolyl hydroxylase primary antibodies
DescriptionMouse polyclonal to PHD4/prolyl hydroxylase
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Full length protein corresponding to Human PHD4/prolyl hydroxylase aa 1-563.
MAAAAVTGQRPETAAAEEASRPQWAPPDHCQAQAAAGLGDGEDAPVRPLC KPRGICSRAYFLVLMVFVHLYLGNVLALLLFVHYSNGDESSDPGPQHRAQ GPGPEPTLGPLTRLEGIKVGHERKVQLVTDRDHFIRTLSLKPLLFEIPGF LTDEECRLIIHLAQMKGLQRSQILPTEEYEEAMSTMQVSQLDLFRLLDQN RDGHLQLREVLAQTRLGNGWWMTPESIQEMYAAIKADPDGDGVLSLQEFS NMDLRDFHKYMRSHKAESSELVRNSHHTWLYQGEGAHHIMRAIRQRVLRL TRLSPEIVELSEPLQVVRYGEGGHYHAHVDSGPVYPETICSHTKLVANES VPFETSCRQVSPNWGLPSILRPGTPMTQAQPCTVGVPLGMGPGDHWVIPV SPWEHPQLGTCSVPPLPYSYMTVLFYLNNVTGGGETVFPVADNRTYDEMS LIQDDVDLRDTRRHCDKGNLRVKPQQGTAVFWYNYLPDGQGWVGDVDDYS LHGGCLVTRGTKWIANNWINVDPSRARQALFQQEMARLAREGGTDSQPEW ALDRAYRDARVEL
- PHD4/prolyl hydroxylase transfected 293T cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.4
Constituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab169483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.|
FunctionCatalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
Tissue specificityWidely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts.
Sequence similaritiesContains 2 EF-hand domains.
Contains 1 Fe2OG dioxygenase domain.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- EGLN4 antibody
- HIF PH4 antibody
- HIF Prolyl Hydroxylase 4 antibody
All lanes : Anti-PHD4/prolyl hydroxylase antibody (ab169483) at 1 µg/ml
Lane 1 : PHD4/prolyl hydroxylase transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 63 kDa
ab169483 has not yet been referenced specifically in any publications.