Overview

  • Product name

    Phenylalanine Assay Kit
  • Detection method

    Fluorescent
  • Sample type

    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type

    Quantitative
  • Sensitivity

    > 2 µM
  • Range

    2 µM - 20 µM
  • Assay time

    0h 30m
  • Product overview

    Phenylalanine Assay Kit ab83376 provides a quick, simple, accurate method for quantifying phenylalanine in biological samples.


    In the phenylalanine assay protocol, phenylalanine is reductively deaminated with the simultaneous formation of NADH which reacts with our fluorescent probe to generate fluorescence at Ex/Em=535/587 nm. The assay is linear in the range from 0.1 to 1.0 nmol (2-20 µM) of Phenylalanine.


    Phenylalanine assay protocol summary:
    - add samples and standards to wells
    - add reaction mix and incubate for 20 min
    - analyze with a microplate reader

  • Notes

    L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Developer (lyophilized) Red 1 vial
    Enzyme Mix (lyophilized) Green 1 vial
    Phenyalanine Standard (1.0 µmol) (lyophilized) Yellow 1 vial
    Phenylalanine Assay Buffer WM 1 x 25ml
    Tyrosinase (lyophilized) Blue 1 vial
  • Research areas

  • Relevance

    L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin. Errors in PHE metabolism lead to phenylketonuria or PKU which can have dire consequences.
  • Alternative names

    • Phe

Associated products

Images

  • Phenylalanine measured in cell lysates showing quantities (micromoles) per million of cells tested.

  • Phenylalanine measured in biofluids showing concentration in micromolar.

  • Phenylalanine fluorimetric standard curve: mean of duplicates (+/- SD) with background readings subtracted

Protocols

References

ab83376 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer

Thank you for contacting us.
The enzyme used in the assay can react with tyrosine and methionine as well as
phenylalanine. Serum methionine concentrations are generally low enough to be
insignificant in this assay. Tyrosine concentrations may interfere. If tyrosine interference is a concern, add 5 μl tyrosinase to the samples and preincubate for 10 minutes before
performing the assay to remove tyrosine interference.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank youfor your inquiry.

Please excuse the delay in my answer. this kit was not tested in-house and I was in contact with the lab that tested it.

Unfortunately, actual data is proprietary information.

But I was assured that the kitis suitable for mouse samples.

We do not know of any publications using this kit at the moment, but will add them to the datasheet as soon as we know of any.

I would like to reassure you that ab83376 is guaranteed for mouse samples by our Abpromise guarantee:

https://www.abcam.com/abpromise

I am sorry that I could not provide all the answers on this occasion and hope this information is nevertheless helpful.

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Question
Answer

Vielen Dank für Ihren Anruf.

Der im Phenylalanin-AssayKit ab83376verwendete Fluoreszenzfarbstoff (pico-probe) unterliegt zwar demBetriebsgeheimnis; allerdings wird er letztendlich in Resorufin umgewandelt - also genau, wie Sie schon vermutet hatten.

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Answer

Thank you for contacting Abcam. We have not tested if this kit can differentiate between the two forms of phenyl alanine, that does not mean it cannot, it just means that we cannot guaranteed that it will. If there is anything else I can help you with, please let me know.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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