Key features and details
- Rabbit polyclonal to PHF1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PHF1 antibody
See all PHF1 primary antibodies
DescriptionRabbit polyclonal to PHF1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog
- 293T cell lysate, human stomach tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab66275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 34, (36), 48 kDa (predicted molecular weight: 62 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
FunctionTranscriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex.
Tissue specificityHighest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.
Sequence similaritiesContains 2 PHD-type zinc fingers.
Cellular localizationNucleus. Localizes specifically to the promoters of numerous target genes.
- Information by UniProt
- hPCl1 antibody
- MTF2 L2 antibody
- MTF2L 2 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling PHF1 with ab66275 at 4-8µg/ml. Arrows indicate positively labelled surface mucous cells and epithelial cells of the fundic gland. Magnification: 400X.
Anti-PHF1 antibody (ab66275) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 62 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 34 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Left hand lane: molecular marker
Gel concentration: 12%
ab66275 has been referenced in 3 publications.
- Park J et al. A 3D human triculture system modeling neurodegeneration and neuroinflammation in Alzheimer's disease. Nat Neurosci 21:941-951 (2018). Human . PubMed: 29950669
- Nie S et al. Bilateral Implantation of Shear Stress Modifier in ApoE Knockout Mouse Induces Cognitive Impairment and Tau Abnormalities. Front Aging Neurosci 10:303 (2018). PubMed: 30337867
- Moussaud S et al. Alpha-synuclein and tau: teammates in neurodegeneration? Mol Neurodegener 9:43 (2014). PubMed: 25352339