Key features and details
- Rabbit polyclonal to PHF1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PHF1 antibody
See all PHF1 primary antibodies
DescriptionRabbit polyclonal to PHF1
SpecificityThis antibody is specific to PHD finger protein 1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Cynomolgus monkey, Macaque monkey
- This antibody gave a positive signal in Human Heart Tissue Lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab80042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionTranscriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex.
Tissue specificityHighest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.
Sequence similaritiesContains 2 PHD-type zinc fingers.
Cellular localizationNucleus. Localizes specifically to the promoters of numerous target genes.
- Information by UniProt
- hPCl1 antibody
- MTF2 L2 antibody
- MTF2L 2 antibody
Anti-PHF1 antibody (ab80042) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Additional bands at: 50 kDa (possible isoform)
Exposure time: 3 minutes
This antibody is predicted to cross react with both isoform 1 (50-kDa) and isoform 2 (62-kDa) of PHF1. We believe that the band observed at 50-kDa corresponds to isoform 1.
ICC/IF image of ab80042 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80042, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) HeLa cells at 1µg/ml.
ab80042 has been referenced in 3 publications.
- Zhang R et al. Aquaporin 4 deletion exacerbates brain impairments in a mouse model of chronic sleep disruption. CNS Neurosci Ther N/A:N/A (2019). PubMed: 31364823
- Qu Y et al. PHF1 is required for chromosome alignment and asymmetric division during mouse meiotic oocyte maturation. Cell Cycle 17:2447-2459 (2018). PubMed: 30382790
- Liu R et al. PHD finger protein 1 (PHF1) is a novel reader for histone H4R3 symmetric dimethylation and coordinates with PRMT5-WDR77/CRL4B complex to promote tumorigenesis. Nucleic Acids Res 46:6608-6626 (2018). PubMed: 29846670