Overview

  • Product name

  • Description

    Rabbit polyclonal to PHF1
  • Host species

    Rabbit
  • Specificity

    This antibody is specific to PHD finger protein 1
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Cynomolgus monkey, Macaque monkey
  • Immunogen

    Synthetic peptide within Human PHF1 aa 150-250 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: O43189
    (Peptide available as ab88938)

  • Positive control

    • This antibody gave a positive signal in Human Heart Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function

    Transcriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex.
  • Tissue specificity

    Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.
  • Sequence similarities

    Contains 2 PHD-type zinc fingers.
  • Cellular localization

    Nucleus. Localizes specifically to the promoters of numerous target genes.
  • Information by UniProt
  • Database links

  • Alternative names

    • hPCl1 antibody
    • MTF2 L2 antibody
    • MTF2L 2 antibody
    • MTF2L2 antibody
    • PCL 1 antibody
    • PCL1 antibody
    • PHD finger protein 1 antibody
    • PHD finger protein1 antibody
    • PHF 1 antibody
    • PHF 2 antibody
    • Phf1 antibody
    • PHF1_HUMAN antibody
    • PHF2 antibody
    • Plc 1 antibody
    • Plc1 antibody
    • Polycomb like protein 1 antibody
    • Polycomb like protein1 antibody
    • Polycomb-like protein 1 antibody
    • Protein PHF1 antibody
    • T complex testis expressed 3 antibody
    • T complex testis expressed3 antibody
    • Tctex 3 antibody
    • Tctex3 antibody
    see all

Images

  • Anti-PHF1 antibody (ab80042) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 62 kDa
    Observed band size: 62 kDa
    Additional bands at: 50 kDa (possible isoform)


    Exposure time: 3 minutes


    This antibody is predicted to cross react with both isoform 1 (50-kDa) and isoform 2 (62-kDa) of PHF1. We believe that the band observed at 50-kDa corresponds to isoform 1.
  • ICC/IF image of ab80042 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80042, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) HeLa cells at 1µg/ml.

References

This product has been referenced in:

  • Zhang R  et al. Aquaporin 4 deletion exacerbates brain impairments in a mouse model of chronic sleep disruption. CNS Neurosci Ther N/A:N/A (2019). Read more (PubMed: 31364823) »
  • Qu Y  et al. PHF1 is required for chromosome alignment and asymmetric division during mouse meiotic oocyte maturation. Cell Cycle 17:2447-2459 (2018). Read more (PubMed: 30382790) »
See all 3 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab80042.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up