Recombinant Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PHF1 antibody [EPR14222] - BSA and Azide free -
Description
Rabbit monoclonal [EPR14222] to PHF1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human PHF1 aa 300-400. The exact sequence is proprietary.
Database link: O43189 -
Positive control
- IHC-P: Human pancreas tissue.
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General notes
Ab232601 is the carrier-free version of ab184951. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab232601 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14222 -
Isotype
IgG
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab232601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 62 kDa. | |
| ICC/IF | Use at an assay dependent concentration. |
Images
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![Flow Cytometry - Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)](https://www.abcam.com/ps/products/232/ab232601/Images/ab232601-4-ab184951217629ab184951FL.jpg)
Flow Cytometry - Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)Flow Cytometric analysis of 293 cells (2% paraformaldehyde-fixed) labeling PHF1 with ab184951 at 1/160 dilution (red) mpared to a negative control antibody (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184951).
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![Immunocytochemistry/ Immunofluorescence - Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)](https://www.abcam.com/ps/products/232/ab232601/Images/ab232601-3-ab184951217626ab184951IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)Immunofluorescent analysis of MCF7 cells (4% paraformaldehyde-fixed) labeling PHF1 with ab184951 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184951).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)](https://www.abcam.com/ps/products/232/ab232601/Images/ab232601-2-ab184951217624ab184951d.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling PHF1 with ab184951 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184951).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)](https://www.abcam.com/ps/products/232/ab232601/Images/ab232601-308678-23260133.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling PHF1 with ab184951 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184951).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Datasheets and documents
References
ab232601 has not yet been referenced specifically in any publications.
