Key features and details
- Rabbit polyclonal to PHF10
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PHF10 antibody
See all PHF10 primary antibodies
DescriptionRabbit polyclonal to PHF10
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to a region within amino acids 243-459 of Human PHF10 (UniProt ID: Q8WUB8).
- HeLa whole cell lysate (ab150035). Mouse colon lysate. HeLa cells. HeLa xenograft.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 56 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionInvolved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Sequence similaritiesBelongs to the SAYP family.
Contains 2 PHD-type zinc fingers.
- Information by UniProt
- BAF45a antibody
- BRG1-associated factor 45a antibody
- FLJ10975 antibody
Anti-PHF10 antibody (ab154637) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 56 kDa
10% SDS PAGE
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling PHF10 with ab154637 at a 1/500 dilution. Alpha-tubulin filaments were labeled (red) at 1/2000.
Anti-PHF10 antibody (ab154637) at 1/1000 dilution + mouse colon lysate/ extract at 50 µg
Predicted band size: 56 kDa
10 % SDS-PAGE
Immunohistochemical analysis of paraffin embedded Hela xenograft labeling PHF10 with ab154637 at a 1/500 dilution.
ab154637 has been referenced in 1 publication.
- Anbunathan H et al. Integrative Copy Number Analysis of Uveal Melanoma Reveals Novel Candidate Genes Involved in Tumorigenesis Including a Tumor Suppressor Role for PHF10/BAF45a. Clin Cancer Res 25:5156-5166 (2019). PubMed: 31227497