Overview

  • Product name
  • Description
    Rabbit polyclonal to PHF10
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 243-459 of Human PHF10 (UniProt ID: Q8WUB8).

  • Positive control
    • HeLa whole cell lysate (ab150035). Mouse colon lysate. HeLa cells. HeLa xenograft.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
  • Sequence similarities
    Belongs to the SAYP family.
    Contains 2 PHD-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAF45a antibody
    • BRG1-associated factor 45a antibody
    • FLJ10975 antibody
    • MGC111009 antibody
    • PHD finger protein 10 antibody
    • PHD finger protein 10, isoform a antibody
    • PHD zinc finger protein XAP135 antibody
    • Phf10 antibody
    • PHF10_HUMAN antibody
    • XAP135 antibody
    see all

Images

  • Anti-PHF10 antibody (ab154637) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 56 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling PHF10 with ab154637 at a 1/500 dilution. Alpha-tubulin filaments were labeled (red) at 1/2000.
  • Anti-PHF10 antibody (ab154637) at 1/1000 dilution + mouse colon lysate/ extract at 50 µg

    Predicted band size: 56 kDa



    10 % SDS-PAGE
  • Immunohistochemical analysis of paraffin embedded Hela xenograft labeling PHF10 with ab154637 at a 1/500 dilution.

References

ab154637 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab154637.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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