Overview

  • Product name

  • Description

    Mouse polyclonal to PHF10
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-410 of Human PHF10

  • Positive control

    • HeLa cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa). This antibody has only been tested against a recombinant cell lysate.

Target

  • Function

    Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
  • Sequence similarities

    Belongs to the SAYP family.
    Contains 2 PHD-type zinc fingers.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • BAF45a antibody
    • BRG1-associated factor 45a antibody
    • FLJ10975 antibody
    • MGC111009 antibody
    • PHD finger protein 10 antibody
    • PHD finger protein 10, isoform a antibody
    • PHD zinc finger protein XAP135 antibody
    • Phf10 antibody
    • PHF10_HUMAN antibody
    • XAP135 antibody
    see all

Images

  • All lanes : Anti-PHF10 antibody (ab68114) at 1/500 dilution

    Lane 1 : PHF10 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugated at 1/2500 dilution

    Predicted band size: 47 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescence analysis of HeLa cells labelled with purified ab68114 at 10 µg/ml followed by fluorescein-conjugated secondary antibody. Cells were fixed in 4% paraformaldehyde and permeabilised with 0.1% Triton X-100.

References

ab68114 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab68114.
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