Key features and details
- Mouse polyclonal to PHF10
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PHF10 antibody
See all PHF10 primary antibodies
DescriptionMouse polyclonal to PHF10
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Full length protein, corresponding to amino acids 1-410 of Human PHF10
- HeLa cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab68114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa). This antibody has only been tested against a recombinant cell lysate.|
FunctionInvolved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Sequence similaritiesBelongs to the SAYP family.
Contains 2 PHD-type zinc fingers.
- Information by UniProt
- BAF45a antibody
- BRG1-associated factor 45a antibody
- FLJ10975 antibody
All lanes : Anti-PHF10 antibody (ab68114) at 1/500 dilution
Lane 1 : PHF10 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugated at 1/2500 dilution
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Immunofluorescence analysis of HeLa cells labelled with purified ab68114 at 10 µg/ml followed by fluorescein-conjugated secondary antibody. Cells were fixed in 4% paraformaldehyde and permeabilised with 0.1% Triton X-100.
ab68114 has not yet been referenced specifically in any publications.