Anti-PHF10 antibody (ab80939)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PHF10 antibody
See all PHF10 primary antibodies -
Description
Rabbit polyclonal to PHF10 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Cat, Dog
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Immunogen
Synthetic peptide corresponding to Human PHF10 aa 290-339 (internal sequence).
Sequence:PELPALDSDGDSDDGEDGRGDEKRKNKGTSDSSSGNVSEGESPPDSQEDS
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Positive control
- Fetal lung lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab80939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. Titre using peptide based assay: 1/312500. |
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| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
Target
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Function
Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. -
Sequence similarities
Belongs to the SAYP family.
Contains 2 PHD-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 55274 Human
- Entrez Gene: 72057 Mouse
- Entrez Gene: 292404 Rat
- Omim: 613069 Human
- SwissProt: Q8WUB8 Human
- SwissProt: Q9D8M7 Mouse
- SwissProt: Q4V7A6 Rat
- Unigene: 435933 Human
see all -
Alternative names
- BAF45a antibody
- BRG1-associated factor 45a antibody
- FLJ10975 antibody
see all
Images
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Western blot - Anti-PHF10 antibody (ab80939)Anti-PHF10 antibody (ab80939) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal lung lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Gel concentration: 12%
Protocols
Datasheets and documents
References
ab80939 has not yet been referenced specifically in any publications.
